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Synonyms
RAP1GA1; USP31
Plasmid
pcDNA5 FRT/TO BirA R118G-USP48-HA
Parent Plasmid
pcDNA5 FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP48-HA
DU Number
DU49970
Genbank
NM_032236.5 GI:76257390
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCCCCGCGGCTGCAGCTGGAGAAGGCGGCCTGGCGCTGGGCGGAGACGGTGCGGCCCGAGGAGGTGTCGCAGGAGCACATCGAGACCGCTTACCGCATCTGGCTGGAGCCCTGCATTCGCGGCGTGTGCAGACGAAACTGCAAAGGAAATCCGAATTGCTTGGTTGGTATTGGTGAGCATATTTGGTTAGGAGAAATAGATGAAAATAGTTTTCATAACATCGATGATCCCAACTGTGAGAGGAGAAAAAAGAACTCATTTGTGGGCCTGACTAACCTTGGAGCCACTTGTTATGTCAACACATTTCTTCAAGTGTGGTTTCTCAACTTGGAGCTTCGGCAGGCACTCTACTTATGTCCAAGCACTTGTAGTGACTACATGCTGGGAGACGGCATCCAAGAAGAAAAAGATTATGAGCCTCAAACAATTTGTGAGCATCTCCAGTACTTGTTTGCCTTGTTGCAAAACAGTAATAGGCGATACATTGATCCATCAGGATTTGTTAAAGCCTTGGGCCTGGACACTGGACAACAGCAGGATGCTCAAGAATTTTCAAAGCTCTTTATGTCTCTATTGGAAGATACTTTGTCTAAACAAAAGAATCCAGATGTGCGCAATATTGTTCAACAGCAGTTCTGTGGAGAATATGCCTATGTAACTGTTTGCAACCAGTGTGGCAGAGAGTCTAAGCTTTTGTCAAAATTTTATGAGCTGGAGTTAAATATCCAAGGCCACAAACAGTTAACAGATTGTATCTCGGAATTTTTGAAGGAAGAAAAATTAGAAGGAGACAATCGCTATTTTTGCGAGAACTGTCAAAGCAAACAGAATGCAACAAGAAAGATTCGACTTCTTAGCCTTCCTTGCACTCTGAACTTGCAGCTAATGCGTTTTGTCTTTGACAGGCAAACTGGACATAAGAAAAAGCTGAATACCTACATTGGCTTCTCAGAAATTTTGGATATGGAGCCTTATGTGGAACATAAAGGTGGGTCCTACGTGTATGAACTCAGCGCAGTCCTCATACACAGAGGAGTGAGTGCTTATTCTGGCCACTACATCGCCCACGTGAAAGATCCACAGTCTGGTGAATGGTATAAGTTTAATGATGAAGACATAGAAAAGATGGAGGGGAAGAAATTACAACTAGGGATTGAGGAAGATCTAGCAGAACCTTCTAAGTCTCAGACACGTAAACCCAAGTGTGGCAAAGGAACTCATTGCTCTCGAAATGCATATATGTTGGTTTATAGACTGCAAACTCAAGAAAAGCCCAACACTACTGTTCAAGTTCCAGCCTTTCTTCAAGAGCTGGTAGATCGGGATAATTCCAAATTTGAGGAGTGGTGTATTGAAATGGCTGAGATGCGTAAGCAAAGTGTGGATAAAGGAAAAGCAAAACACGAAGAGGTTAAGGAGCTGTACCAAAGGTTACCTGCTGGAGCTGAGCCCTATGAGTTTGTCTCTCTGGAATGGCTGCAAAAGTGGTTGGATGAATCAACACCTACCAAACCTATTGATAATCACGCTTGCCTGTGTTCCCATGACAAGCTTCACCCGGATAAAATATCAATTATGAAGAGGATATCTGAATATGCAGCTGACATTTTCTATAGTAGATATGGAGGAGGTCCAAGACTAACTGTGAAAGCCCTGTGTAAGGAATGTGTAGTAGAACGTTGTCGCATATTGCGTCTGAAGAACCAACTAAATGAAGATTATAAAACTGTTAATAATCTGCTGAAAGCAGCAGTAAAGGGCAGCGATGGATTTTGGGTGGGGAAGTCCTCCTTGCGGAGTTGGCGCCAGCTAGCTCTTGAACAGCTGGATGAGCAAGATGGTGATGCAGAACAAAGCAACGGAAAGATGAACGGTAGCACCTTAAATAAAGATGAATCAAAGGAAGAAAGAAAAGAAGAGGAGGAATTAAATTTTAATGAAGATATTCTGTGTCCACATGGTGAGTTATGCATATCTGAAAATGAAAGAAGGCTTGTTTCTAAAGAGGCTTGGAGCAAACTGCAGCAGTACTTTCCAAAGGCTCCTGAGTTTCCAAGTTACAAAGAGTGCTGTTCACAGTGCAAGATTTTAGAAAGAGAAGGGGAAGAAAATGAAGCCTTACATAAGATGATTGCAAACGAGCAAAAGACTTCTCTCCCAAATTTGTTCCAGGATAAAAACAGACCGTGTCTCAGTAACTGGCCAGAGGATACGGATGTCCTCTACATCGTGTCTCAGTTCTTTGTAGAAGAGTGGCGGAAATTTGTTAGAAAGCCTACAAGATGCAGCCCTGTGTCATCAGTTGGGAACAGTGCTCTTTTGTGTCCCCACGGGGGCCTCATGTTTACATTTGCTTCCATGACCAAAGAAGATTCTAAACTTATAGCTCTCATATGGCCCAGTGAGTGGCAAATGATACAAAAGCTCTTTGTTGTGGATCATGTAATTAAAATCACGAGAATTGAAGTGGGAGATGTAAACCCTTCAGAAACACAGTATATTTCTGAGCCCAAACTCTGTCCAGAATGCAGAGAAGGCTTATTGTGTCAGCAGCAGAGGGACCTGCGTGAATACACTCAAGCCACCATCTATGTCCATAAAGTTGTGGATAATAAAAAGGTGATGAAGGATTCGGCTCCGGAACTGAATGTGAGTAGTTCTGAAACAGAGGAGGACAAGGAAGAAGCTAAACCAGATGGAGAAAAAGATCCAGATTTTAATCAAAGCAATGGTGGAACAAAGCGGCAAAAGATATCCCATCAAAATTATATAGCCTATCAAAAGCAAGTTATTCGCCGAAGTATGCGACATAGAAAAGTTCGTGGTGAGAAAGCACTTCTCGTTTCTGCTAATCAGACGTTAAAAGAATTGAAAATTCAGATCATGCATGCATTTTCAGTTGCTCCTTTTGACCAGAATTTGTCAATTGATGGAAAGATTTTAAGTGATGACTGTGCCACCCTAGGCACCCTTGGCGTCATTCCTGAATCTGTCATTTTATTGAAGGCTGATGAACCAATTGCAGATTATGCTGCAATGGATGATGTCATGCAAGTTTGTATGCCAGAAGAAGGGTTTAAAGGTACTGGTCTTCTTGGACATGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMAPRLQLEKAAWRWAETVRPEEVSQEH
IETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDD
PNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDY
MLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTG
QQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCG
RESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQ
NATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYV
EHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKM
EGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPN
TTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQ
RLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIM
KRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKT
VNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGS
TLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQ
YFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDK
NRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCP
HGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDV
NPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMK
DSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAY
QKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSI
DGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGF
KGTGLLGHAAAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMAPRLQLEKAAWRWAETVRPEEVSQEH
IETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDD
PNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDY
MLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTG
QQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCG
RESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQ
NATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYV
EHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKM
EGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPN
TTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQ
RLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIM
KRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKT
VNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGS
TLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQ
YFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDK
NRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCP
HGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDV
NPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMK
DSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAY
QKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSI
DGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGF
KGTGLLGHAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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