USP35

Expresses USP35 Nter KI guide 2, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets. Targets the sense strand near the start codon of exon 2, designed to knock-in an in-frame GFP tag to the N-terminus of USP35. To be co-transfected along with guide RNA DU48242 and the GFP donor DU48? into a cell-line expressing wild-type Cas9. This guide may also be used to KO by indel formation, screen for by RFLP-AatII/ZraI site loss.