PIAS4 | Ubiquitylation Resource

Plasmid

pcDNA5-FRT/TO-EYFP-PIAS4 (siRNA resistant) deletion SAP

Parent Plasmid

pcDNA5 FRT/TO

Expressed

EYFP-PIAS4 (siRNA resistant) delta V6-R65

DU Number

DU24570

Genbank

BC029874 NM_015897.2

Tag

YFP

Species

Human

Sequence of Insert

View Sequence

AAGATGGCGGCGGAGCTGTACGCCAAGAAGAACTCGGAGCCTGCCCCACA
GCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGG
CCGGCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCC
GTGCTCTACGGAAAGTACTTAAACGGACTGGGACGGTTGCCCGCCAAGAC
CCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGG
ATGAGCTGCTGAAGCCCACCGAAttggttcctCAGAACAACGAGAAGCTT
CAGGAGAGCCCGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGAT
CCGGAACTCCAGGGAACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCC
TGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCG
CCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTA
CTACCCCTCCAATAAGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCA
TCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCGCATCACT
GTCACCTGGGGGAACtatggtaaaAGCTACTCGGTGGCCCTGTACCTGGT
GCGGCAGCTGACCTCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGG
TAAAGCACCCGGAGCTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTT
GATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTG
TCCGCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCG
CCCACCTGCAGTGCTTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAG
AAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCA
GCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACG
CCGACGAGATCGAGTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCC
GAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCC
CTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTG
CCCTGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGG
AAGCCGGGCGCCGATGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTC
GGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGG
GGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCC
TGCTGACCCCGGCCGCACACTCGACgcggccgc

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELYAK
KNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNG
LGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFAL
TPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNH
SYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKS
YSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATT
GVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVC
DKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQ
GAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDL
TLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Silent mutations at L151 V152 P153 (tta gtc cca → ttg gtt cct ) and Y265 G266 K267 (tac ggc aag → tat ggt aaa) plus SAP mutation (delta V6-R65). Clone has an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.

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