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Synonyms
HRIHFB2157, ALS15, CHAP1, DSK2, N4BP4, PLIC2
Plasmid
pMA UBQLN2 Nter Puro KO
Parent Plasmid
pMA
DU Number
DU48272
Genbank
NM_013444.3
Species
Human
Sequence of Insert
View Sequence
GTCTCACCCTCTCTGCCTCGCCTGCTCTGTACTGTTCTGTTCTCTTCTCTCTCTCCCTTTGTTTCTCTCTCTCCCTTTTTTCTTTTCAGTGCAGAAGTTTTTAATTTTGACCGAGCCAAATCTATCAATCTAGGCCTGGCGCCGCGTCAGCAGAGGGGGCGGGGAGGCGAGCGCGGAACTGGGGGAGGGGAAGGGGCGGGGATCAGCAGGCGGAGCGGCTGCCAGAGTTGCTGGGAGTGCGCGCGGTCGGATCACAAGGCGGCGGCGGAGGAGGCCCAGAGACCGGAGCGCGGAGACCTCAGCCAGCGGCCTACGCCCAGGCCTTTCTCCACCGGAGGACCAGGGAACCGCAGTCTTCATCACAGAGGTACCGTGCTCCGCGCTCCCCGCCTGACCCGGCCCAGCCCGCTGCGGCGGTGCCTCCTTCCTTCCTCCTTCCCTCGCGCTCTCTCTTTCGCCCGCCCGCGCCTTCCCTGCCCGCCTGCGTCACCGCGGCCGCCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGCaGAaAAcGGaGAatcCtcaGGCCCCCCGCGCCCCTCCCGCGGCCCTGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAATCATCAAAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTGCCCGAGAACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTTCAAATCCCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAAAAGATCAAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTTCACCTTGTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCCTAGCAATGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTAACTCCACACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTGGGAGGACTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTCTGAGCTCCAGAGCCAGATGCAGCAGCAGCTTA
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR donor vector designed to KO UBQLN2 by introducing a Puromycin marker (with stop codon) to the N-terminus (Exon1). Contains several silent mutations to prevent binding of guide RNA. To be used in conjunction with DU48238. Possibility of NMD, donor could be improved by adding a polyA signal after the Puro marker.
100042 R 1000 1001 1047 19007
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