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Plasmid
pcDNA5-FRT/TO-GFP-USP21
Parent Plasmid
pcDNA5D FRT/TO GFP
Expressed
GFP-USP21
DU Number
DU24494
Genbank
NM_001014443.2
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GTCGACATGCCCCAGGCCTCTGAGCACCGCCTGGGCCGTACCCGAGAGCCACCTGTTAATATCCAGCCCCGAGTGGGATCCAAGCTACCATTTGCCCCCAGGGCCCGCAGCAAGGAGCGCAGAAACCCAGCCTCTGGGCCAAACCCCATGTTACGACCTCTGCCTCCCCGGCCAGGTCTGCCCGATGAACGGCTCAAGAAACTGGAGCTGGGACGGGGACGGACCTCAGGCCCTCGTCCCAGAGGCCCCCTTCGAGCAGATCATGGGGTTCCCCTGCCTGGCTCACCACCCCCAACAGTGGCTTTGCCTCTCCCATCTCGGACCAACTTAGCCCGTTCCAAGTCTGTGAGCAGTGGGGACTTGCGTCCAATGGGGATTGCCTTGGGAGGGCACCGTGGCACCGGAGAGCTTGGGGCTGCACTGAGCCGCTTGGCCCTCCGGCCTGAGCCACCCACTTTGAGACGTAGCACTTCTCTCCGCCGCCTAGGGGGCTTTCCTGGACCCCCTACCCTGTTCAGCATACGGACAGAGCCCCCTGCTTCCCATGGCTCCTTCCACATGATATCCGCCCGGTCCTCTGAGCCTTTCTACTCTGATGACAAGATGGCTCATCACACACTCCTTCTGGGCTCTGGTCATGTTGGCCTTCGAAACCTGGGAAACACGTGCTTCCTGAATGCTGTGCTGCAGTGTCTGAGCAGCACTCGACCTCTTCGGGACTTCTGTCTGAGAAGGGACTTCCGGCAAGAGGTGCCTGGAGGAGGCCGAGCCCAAGAGCTCACTGAAGCCTTTGCAGATGTGATTGGTGCCCTCTGGCACCCTGACTCCTGCGAAGCTGTGAATCCTACTCGATTCCGAGCTGTCTTCCAGAAATATGTTCCCTCCTTCTCTGGATACAGCCAGCAGGATGCCCAAGAGTTCCTGAAGCTCCTCATGGAGCGGCTACACCTTGAAATCAACCGCCGAGGCCGCCGGGCTCCACCGATACTTGCCAATGGTCCAGTTCCCTCTCCACCCCGCCGAGGAGGGGCTCTGCTAGAAGAACCTGAGTTAAGTGATGATGACCGAGCCAACCTAATGTGGAAACGTTACCTGGAGCGAGAGGACAGCAAGATTGTGGACCTGTTTGTGGGCCAGTTGAAAAGTTGTCTCAAGTGCCAGGCCTGTGGGTATCGCTCCACGACCTTCGAGGTTTTTTGTGACCTGTCCCTGCCCATCCCCAAGAAAGGATTTGCTGGGGGCAAGGTGTCTCTGCGGGATTGTTTCAACCTTTTCACTAAGGAAGAAGAGCTAGAGTCGGAGAATGCCCCAGTGTGTGACCGATGTCGGCAGAAAACTCGAAGTACCAAAAAGTTGACAGTACAAAGATTCCCTCGAATCCTCGTGCTCCATCTGAATCGATTTTCTGCCTCCCGAGGCTCCATCAAAAAAAGTTCAGTAGGTGTAGACTTTCCACTGCAGCGACTGAGCCTAGGGGACTTTGCCAGTGACAAAGCCGGAAGTCCTGTATACCAGCTGTATGCCCTTTGCAACCACTCAGGCAGCGTCCACTATGGCCACTACACAGCCCTGTGCCGGTGCCAGACTGGTTGGCATGTCTACAATGACTCTCGTGTCTCCCCTGTCAGTGAAAACCAGGTGGCATCCAGCGAGGGCTACGTGCTGTTCTACCAACTGATGCAGGAGCCACCCCGGTGCCTGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LDMPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASGPNPM
LRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTV
ALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEP
PTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDD
KMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQE
VPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYS
QQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPE
LSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFC
DLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTK
KLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAG
SPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASS
EGYVLFYQLMQEPPRCL*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LDMPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASGPNPM
LRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTV
ALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEP
PTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDD
KMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQE
VPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYS
QQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPE
LSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFC
DLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTK
KLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAG
SPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASS
EGYVLFYQLMQEPPRCL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 41455. Silent mutation: t177c derived from source clone
100042 R 1000 1001 1047 19007
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