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USP45

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Plasmid
pcDNA5frtTO GFP NLS USP45 61-end C199A
Parent Plasmid
pcDNA5 FRT/TO GFP NLS
Expressed
GFP-NLS-USP45 61-end C199A
DU Number
DU48244
Genbank
NP_001073950.1
Tag
GFP
NLS
Species
Human
Sequence of Insert
AGATCTATGGTTTGCTCAGAATGTTTAAAAGAAAGAAGATTCTATGATGGGCAGCTAGTACTTACTTCTGATATTTGGTTGTGCCTCAAGTGTGGCTTCCAGGGATGTGGTAAAAACTCAGAAAGCCAACATTCATTGAAGCACTTTAAGAGTTCCAGAACAGAGCCCCATTGTATTATAATTAATCTGAGCACATGGATTATATGGTGTTATGAATGTGATGAAAAATTATCAACGCATTGTAATAAGAAGGTTTTGGCTCAGATAGTTGATTTTCTCCAGAAACATGCTTCTAAAACACAAACAAGTGCATTTTCTAGAATCATGAAACTTTGTGAAGAAAAATGTGAAACAGATGAAATACAGAAGGGAGGAAAATGCAGAAATTTATCTGTAAGAGGAATTACAAATTTAGGAAATACTGCCTTTTTTAATGCAGTCATGCAGAACTTGGCACAGACTTATACTCTTACTGATCTGATGAATGAGATCAAAGAAAGTAGTACAAAACTCAAGATTTTTCCTTCCTCAGACTCTCAGCTGGACCCATTGGTGGTGGAACTTTCAAGGCCTGGACCACTGACTTCTGCCTTGTTCCTGTTTCTTCACAGCATGAAGGAGACTGAAAAAGGACCACTTTCTCCTAAAGTTCTTTTTAATCAGCTTTGTCAGAAGGCACCTCGATTTAAAGATTTCCAGCAACAGGACAGTCAGGAGCTTCTTCATTATCTTCTGGATGCAGTGAGGACAGAAGAAACAAAGCGAATACAAGCTAGCATTCTAAAAGCATTTAACAACCCAACTACTAAAACTGCTGATGATGAAACTAGAAAAAAAGTCAAAGCATATGGAAAAGAAGGTGTGAAAATGAACTTCATAGATCGGATCTTTATTGGTGAATTAACTAGCACGGTCATGTGTGAAGAATGTGCAAATATCTCCACGGTGAAAGATCCATTCATTGATATTTCACTTCCTATAATAGAAGAAAGGGTTTCAAAACCTTTACTTTGGGGAAGAATGAATAAATATAGAAGTTTACGGGAGACAGATCATGATCGATACAGTGGCAATGTTACTATAGAAAATATTCATCAACCTAGAGCTGCCAAGAAGCATTCTTCATCTAAAGATAAGAGTCAACTAATTCATGACCGAAAATGTATTAGAAAATTGTCATCTGGAGAAACTGTCACATACCAGAAAAATGAAAACCTTGAAATGAATGGGGATTCTTTAATGTTTGCCAGCCTCATGAATTCTGAGTCACGTCTGAATGAAAGCCCTACTGATGACAGTGAAAAAGAAGCCAGCCATTCTGAAAGCAATGTTGATGCTGACAGTGAGCCTTCAGAATCTGAAAGTGCTTCAAAGCAGACTGGGCTGTTCAGATCCAGTAGTGGATCCGGTGTGCAGCCAGATGGACCCCTTTACCCTCTGTCAGCAGGTAAACTGCTGTACACCAAGGAGACTGACAGTGGTGATAAGGAAATGGCAGAAGCTATTTCTGAACTTCGTTTGAGCAGCACTGTAACTGGAGATCAAGATTTTGACAGAGAAAATCAGCCACTAAATATTTCAAATAATTTATGTTTTTTGGAGGGGAAGCATTTGAGGTCTTATAGTCCCCAAAATGCTTTTCAGACCCTTTCTCAGAGCTATATAACTACTTCTAAAGAATGTTCAATTCAGTCCTGTCTCTACCAGTTTACATCTATGGAATTACTAATGGGGAATAATAAGCTTCTATGTGAGAATTGTACTAAAAACAAACAGAAGTACCAAGAAGAAACCAGTTTTGCAGAAAAGAAAGTAGAAGGAGTTTATACTAATGCCAGGAAGCAATTGCTCATTTCTGCTGTTCCAGCTGTCCTAATTCTCCACCTGAAAAGATTTCATCAGGCTGGCTTGAGTCTTCGTAAAGTAAACAGACATGTAGATTTTCCACTTATGCTCGATTTAGCACCATTCTGCTCTGCTACTTGTAAGAATGCAAGTGTGGGAGATAAAGTTCTCTACGGTCTCTATGGCATAGTGGAACATAGTGGCTCGATGAGAGAAGGCCACTACACTGCTTATGTGAAAGTGAGAACACCCTCCAGGAAATTATCGGAACATAACACTAAAAAGAAAAATGTGCCTGGTTTGAAAGCGGCTGATAATGAATCAGCAGGCCAGTGGGTCCATGTTAGTGACACTTACTTACAGGTGGTTCCAGAATCAAGAGCACTTAGTGCACAAGCCTACCTTCTTTTCTATGAAAGAGTATTATAAGCGGCCGC

Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKV
GSMVCSECLKERRFYDGQLVLTSDIWLCLKCGFQGCGKNSESQHSLKHFK
SSRTEPHCIIINLSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASKT
QTSAFSRIMKLCEEKCETDEIQKGGKCRNLSVRGITNLGNTAFFNAVMQN
LAQTYTLTDLMNEIKESSTKLKIFPSSDSQLDPLVVELSRPGPLTSALFL
FLHSMKETEKGPLSPKVLFNQLCQKAPRFKDFQQQDSQELLHYLLDAVRT
EETKRIQASILKAFNNPTTKTADDETRKKVKAYGKEGVKMNFIDRIFIGE
LTSTVMCEECANISTVKDPFIDISLPIIEERVSKPLLWGRMNKYRSLRET
DHDRYSGNVTIENIHQPRAAKKHSSSKDKSQLIHDRKCIRKLSSGETVTY
QKNENLEMNGDSLMFASLMNSESRLNESPTDDSEKEASHSESNVDADSEP
SESESASKQTGLFRSSSGSGVQPDGPLYPLSAGKLLYTKETDSGDKEMAE
AISELRLSSTVTGDQDFDRENQPLNISNNLCFLEGKHLRSYSPQNAFQTL
SQSYITTSKECSIQSCLYQFTSMELLMGNNKLLCENCTKNKQKYQEETSF
AEKKVEGVYTNARKQLLISAVPAVLILHLKRFHQAGLSLRKVNRHVDFPL
MLDLAPFCSATCKNASVGDKVLYGLYGIVEHSGSMREGHYTAYVKVRTPS
RKLSEHNTKKKNVPGLKAADNESAGQWVHVSDTYLQVVPESRALSAQAYL
LFYERVL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
61-end, C199A. 3 silent nucleotide changes as compared to the reference sequence; C>T 576 bp, A>T 579 bp and A>G 1590 bp.
100042 R 1000 1001 1047 19007
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