FancI (chicken)/Fancd2 (chicken) | Ubiquitylation Resource

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Plasmid

pET15b 6His TEVb chicken FancI 12x(TGS) chicken fancd2

Parent Plasmid

pET15b original

Expressed

6His TEVb chicken FancI 12x(TGS) chicken fancd2

DU Number

DU49477

Genbank

See Notes

Tag

6His

Species

Chicken

Sequence of Insert

View Sequence

GGATCCACGGGGAGCACTGGTTCAACAGGTTCCACCGGAAGCACAGGAAG
TACTGGTTCTATGGGAATGGTTTCGAAAAGGAAGTTGTCTAAAATTGATG
CTGCTGAAGAGAGCTCCAAGACAGACCTGCAGAGTAGATGCCCAGAAACA
AAGAGGTCACGGATTTCTGATAAGAGGGCCCCTTCCCAAGGTGGATTGGA
GAATGAAGGAGTATTTGAAGAGCTCCTGAGAACATCAGGAATCATACTGA
AAGTTGGGGAAGGACAGAATGAAATAGCTGTGGATCAAACAGCTTTCCAG
AAGAAGCTCCGCGTGGCTCTGGAGAAACACCCATCATACCCAGGTGTTGT
GAATGAGTTCATCAGTGGATTGGAATCTCACATAAAGGACAGAAGTCAGT
TCAAGAACTGCCTCTTGCCATGCACTCCCGCGCGGACTGAGGGGTCAAGG
ACTCTGGTGCACTCATACTGTGAAAGCCTCATTAAGCTGCTGCTTGGAAT
TAAGATCTTACAGCCTGCTGTTGTAACACTGCTGTTGGAAAAGATCCCTG
AATTCTTTTTTGATGTTGTGGGTACCTTTGGAACAAACTTCCCTCGGCTC
ATTGTCAACCAGTTTAAATGGCTCGACGGTCTTCTTGACAGCCAGGATCT
TGTCAAGAAGTTAATGCAGATGCTTAGCGTGTCTCCTGTACCAATCCAGC
ATGATATTATCACCAGTTTACCTGAGATCCTGGAGGATTCCCAGCAAAAT
GAGGTTGCCAGAGAGCTGAGCTGCTTACTGAAACAGGGCAGACGGCTCAC
AGTTCCAATTCTGGATGCTCTTTCTCGTTTGGATCTTGATGCAGAACTTC
TGGCCGAGGTGCGGCAGTCTGCCATGACCATTGTGCCTAGTGTAAAACTG
GAAGATTTGCCTGTGGTCATAAAATTCATTCTCCATAATGTCAAGGCCGC
AGATGCCGTAGAGGTGATTTCTGATCTTCGGAAGAGCTTGGATCTGTCAT
CATGCGTTCTGCCTCTGCAGCTGCTAGGTTCCCAGAGAAAGCTTAAGAGC
CAAGCCCAGGCCAGTTCTTCCATGAGCCAAGTGACCACCAGCCAGAATTG
TGTGAAGCTGCTTTTTGATGTGATCAAGCAAGCTGTAAGATTCCAGAAAG
ATGTCTCTGAAGCTTGGATTAAGGCTATTGAGAACAGCACATCTGTATCT
GACCACAAGGTTCTGGATCTGATCGTTTTGCTGCTAATCCATTCTACAAA
CAGCAAGAACAGGAAGCAAACTGAGAAGGTATTGAGGAGCAAGATTCGCC
TGGGCTGCATGCCAGAGCAGCTGATGCAGAACGCTTTCCAAAATCATTCT
ATGGTCATCAAGGATTTCTTCCCTTCAATCCTATCACTTGCCCAGACGTT
TCTGCACTCTGCACACCCAGCTGTGGTCTCCTTTGGCAGCTGCATGTACA
AACAAGCTTTTGCAGTCTTTGACTCTTACTGCCAGCAGGAGGTTGTATGT
GCTTTGGTCACTCATGTATGCAGTGGAAATGAAACAGAGTTGGATATTTC
TCTGGACGTGCTCACGGATTTGGTGATCCTACACCCATCCCTGCTGCTGC
GCTATGCCACCTTCGTGAAGACCATTTTAGACTCTATGCAGAAACTGAAT
CCATGCCAGATCCGGAAGCTTTTCTACATTCTCAGCACACTAGCATTCAG
CCAGAGACAAGAAGGAAGTTATATTCAGGATGATATGCACATGGTGATCA
GGAAGTGGCTCTCCAGCTCTGTTCCCAACCACAAACAGATGGGGATTATT
GGTGCTGTTACCATGATGGGCAGTGTGGCATTAAAAAGAAATGAAGCAGA
TGGTGGTTTGCTGGAGAGACCTGAGCTGAGCATCGAATGTGATGGGCAGC
TTTCTACACTGCTGGACCTTGTTGGCTTCTGCTGTGAGCAAACACCAGAG
GTCTTGGCTCTGTACTATGATGAACTTGCCAATTTGATTGAGAAGCAGAA
GGGAAATTTGGACCTGCAGCTCCTGGATAAGTTTGGGAAGAGTTTGGTGG
AGGACTTTCCCAATGACTTTGTGGTGGATCTCAGCCCTACAGTGGATGGT
TCCTTTTTGTTCCCAGTGAAGTCCTTGTATAATCTGGATGAAGATGAAAC
TCAGGGAGCAATTGCCATTAACCTCCTACCACTGGTGTCACAGAGTGAGC
CTGGCAGGGTCGCTGATGAAATGAGTAACTCAAGGAAAAGGGTAGTATCA
CCAATATGCCTATCACCCTGTTTCCGATTGCTCAGACTCTACACTGGAGA
ACAAAACAATGGAAGCCTGGAGGAGATTGATGCCTTACTAGGTTGCCCTC
TGTACCTGACTGACCTGGAGGTGGAAGGGAAGCTGGACTCTCTCTCCAAG
CAGGAACGGGAATTTCTATGCTCACTCCTTTTCTATGCACTCAACTGGTT
TCGTGAGGTTGTAAATGCCTTCTGCCAGCAACAAGATGCTGAGATGAAGG
GGAAAGTTCTGACTCGACTACAGAACATCACAGAGCTGCAGAATGTGCTG
GGAAAATGTTTGGCAGCAACCCCTGGCTATGTCCCACCGCCTGCTACTTT
TGATTCTGAAGCCCCAGAGGGAGTACCATCCATTAATGCTGGTGGTCCAG
TGAGAAAAAAAAATGGAAAGAAAAGAAAGTCTGACAGCAGCAAAGCCTGC
AGTGCAGAACGTACTCAGGCAGATGAAAGTTCTGATGGAAACCAGCCTGA
TACTGAGCTCTCCGAGCTGGAGAAGAGTGCTACTGAGAAAGAGACAGGAA
ACCCTCTGGCACAGCTGCAGAGTTATCGCCCCTACTTCCGAGAGCTGGAC
CTGGAAGTGTTCTCTGTACTCCACTGTGGGCTGCTGACAAAGTCTATCCT
GGACACAGAGATGCACACAGAGGCTAGTGAAGTTGTGCAGCTGGGGCCTG
CTGAGCTCTGCTTCCTTCTGGATGACATGTGCTGGAAGCTGGAACATGTG
CTGACCCCAGGCTCCACTAGGAGAGTGCCCTTCTTAAAGGAAAGAGGCAA
CAAGGATGTTGGTTTTTCCCACCTTTGTCAGAGATCCCCAAAGGAAGTTG
CTGTGTGTGTAGTCAAGCTACTAAAACCACTGTGCAATCACATGGAGAAC
ATGCACAATTACTTCCAGACAGTTATACCAAACCAAGGTGTGGTAGATGA
ATCAGGACTGAACATCCAGGAGTACCAGCTGATGTCCTCTTGTTACCACC
AGCTGCTGCTAGCTTTTCGCTTGTTGTTTGCATGGAGTGGTTTTTCTCAG
CATGAGAATTCCAACTTGCTGAGGTCAGCTCTCCAGGTGCTTGCAGACAG
GCTGAAGCCAGGAGAGACAGAGTTCCTTCCTCTTGAAGAGCTGATAAGTG
AGAGTTTCCAATACCTACTGAATTTCCAGGCGAGCATTCCCAGCTTCCAG
TGTGCGTTCATCCTCACTCAGGTCCTGATGGCCATCTCTGAAAAACCAAT
GACTGGCTGGAAGAGAGAGAAAATGGCTTCACTAGCAAAGCAGTTCCTCT
GCCAGTCATGGATGAAGCCTGGTGGGGACCGAGAGAAGGGCAGCCACTTC
AACAGCGCGCTGCATACTTTGCTCTGTGTCTACCTGGAGCACACAGATAA
CATCTTGAAAGCTATAGAAGAAATCTCCAGCGTCGGTGTTCCTGAATTGA
TCAACTCTGCCAAAGATGGATGTTCTTCTACATATCCCACACTGAGCAGA
CAGACATTCCCAGTTTTCTTCAGAGTAATGATGGCTCAGCTAGAGAGTTC
CGTGAAAAGCATCCCAGCTGGGAAACCATCAGACTCAGGCGAGGTGCAAC
TGGAGAAGCTGCTGAAGTGGAACATCGCTGTGCGGAATTTCCATATCCTC
ATTAACTTGGTCAAGGTGTTTGACAGCAGGCCTGTACTCAGCATCTGCCT
GAAGTATGGCCGTCTCTTTGTAGAAGCATTTCTGAAGCTCGCCATGCCTC
TCCTGGACCACAGCTTTAAAAAACATCGGGACGATGTGCAGAGTTTGCTG
AAAACACTGCAGCTGAGTACCAGACAGCTCCATCACATGTGTGGCCATTC
CAAGATTCACCAGGATCTCGGCCTGACCAACCACGTGCCTTTGCTGAAGA
AGTCCCTGGAACAATTTGTGTACCGTGTGAAGGCGATGCTGGCCTTCAAC
CACTGCCAGGAGGCCTTCTGGGTGGGCGTCCTGAAGAACCGGGACCTGCA
GGGGGAAGAAATCCTGTCCCAGGCTTCCGCAGCGCCGGAGGAGGACTCCG
CGGAGGGCTCGGAGGAGGACACGGCGGACAGCGCAGCGGAGGAGCCGGAC
GGAACGGACAGCGACAGCGGCGGGGCGGGGCGCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHENLYFQMAQRILQLAAEGSPERLQEALQGLTEGELGDMVT
RQALRGRETAALLKGIFKGSPCSQQSGVLRRLQVYKHCVSLVESGDLHVG
KVSEIIGLLMLEARQLPGHALAELATLFVEVIKRGSLSNGKSLELFSTVL
TALSNSKESLAYGKGELNGEEFKKQLINTLCSSKWDPQCVIHLANMFRDI
PLSGEELQFVVEKVLRMFSKLDLQEIPPLVYQLLLLSAKGSKKTVLEGII
SFFNQLDKRQKEEQRVPQSADLEVATVPLDQLRHVEGTVILHIVSAINLD
QDIGEELIKHLKTEQQKDPGKALCPFSVSLLLSTAVKHRLQEQIFDFLKT
SITRSCKDLQILQASKFLQDLCPQQYDVTAVILEVVKNSAFGWDHVTQGL
VDLGFSLMESYEPKKSFGGKAAETNLGLSKMPAQQACKLGASILLETFKV
HEPIRSDILEQVLNRVLTKAASPVSHFIDLLSNIVVSAPLVLQNSSSRVT
ETFDNLSFLPIDTVQGLLRAVQPLLKVSMSVRDSLILVLQKAIFSRQLDA
RKAAVAGFLLLLRNFKILGSLTSSQCSQAIGATQVQADVHACYNSAANEA
FCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSIMETLLSQIK
QYYLPQQDLLPPLKLEGCIMAQGDQIFLQEPLAHLLCCIQHCLAWYKSTV
HLCKGAEDEEEEEDVGFEQNFEEMLESVTRRMIKSELEDFELDKSADFSP
SSGVGVKNNIYAIQVMGICEVLIEYNFKIGNFSKNKFEDVLGLFTCYNKL
SEILKEKAGKNKSTLGNRIARSFLSMGFVSTLLTALFRDNAQSHEESLAV
LRSSTEFMRYAVSVALQKVQQLEEMGQTDGPDGQNPEKMFQNLCKITRVL
LWRYTSIPTAVEESGKKKGKSISLLCLEGLLRIFNTMQQLYAARIPQFLQ
ALDITDGDAEEADINVTEKAAFQIRQFQRSLVNQLSSAEDDFNSKETQLL
ITILSTLSKLLDPGSQQFLQFLTWTVKICKENALEDLSCCKGLLTLLFSL
HVLYKSPVSLLRELAQDIHACLGDIDQDVEIESRSHFAIVNVKTAAPTVC
LLVLGQADKVLEEVDWLIKRLTILGSDTSEDSTQASNQTQALEKGVILQL
GTLLTVFHELVQTALPAGSCVDSLLRSLSKTYAILTSLIKHYIQACRSTS
NTVPGRLEKLVKLSGSHLTPQCYSFITYVQNIHSESLSFAEEKKKKKKED
ETAVVSTVMAKVLRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYMKL
STSRDFRINASMLDSVLQEQNTEDAENEPDNNQSGTAEQPDENQEPQKKR
RRKKTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGMVSKRKLS
KIDAAEESSKTDLQSRCPETKRSRISDKRAPSQGGLENEGVFEELLRTSG
IILKVGEGQNEIAVDQTAFQKKLRVALEKHPSYPGVVNEFISGLESHIKD
RSQFKNCLLPCTPARTEGSRTLVHSYCESLIKLLLGIKILQPAVVTLLLE
KIPEFFFDVVGTFGTNFPRLIVNQFKWLDGLLDSQDLVKKLMQMLSVSPV
PIQHDIITSLPEILEDSQQNEVARELSCLLKQGRRLTVPILDALSRLDLD
AELLAEVRQSAMTIVPSVKLEDLPVVIKFILHNVKAADAVEVISDLRKSL
DLSSCVLPLQLLGSQRKLKSQAQASSSMSQVTTSQNCVKLLFDVIKQAVR
FQKDVSEAWIKAIENSTSVSDHKVLDLIVLLLIHSTNSKNRKQTEKVLRS
KIRLGCMPEQLMQNAFQNHSMVIKDFFPSILSLAQTFLHSAHPAVVSFGS
CMYKQAFAVFDSYCQQEVVCALVTHVCSGNETELDISLDVLTDLVILHPS
LLLRYATFVKTILDSMQKLNPCQIRKLFYILSTLAFSQRQEGSYIQDDMH
MVIRKWLSSSVPNHKQMGIIGAVTMMGSVALKRNEADGGLLERPELSIEC
DGQLSTLLDLVGFCCEQTPEVLALYYDELANLIEKQKGNLDLQLLDKFGK
SLVEDFPNDFVVDLSPTVDGSFLFPVKSLYNLDEDETQGAIAINLLPLVS
QSEPGRVADEMSNSRKRVVSPICLSPCFRLLRLYTGEQNNGSLEEIDALL
GCPLYLTDLEVEGKLDSLSKQEREFLCSLLFYALNWFREVVNAFCQQQDA
EMKGKVLTRLQNITELQNVLGKCLAATPGYVPPPATFDSEAPEGVPSINA
GGPVRKKNGKKRKSDSSKACSAERTQADESSDGNQPDTELSELEKSATEK
ETGNPLAQLQSYRPYFRELDLEVFSVLHCGLLTKSILDTEMHTEASEVVQ
LGPAELCFLLDDMCWKLEHVLTPGSTRRVPFLKERGNKDVGFSHLCQRSP
KEVAVCVVKLLKPLCNHMENMHNYFQTVIPNQGVVDESGLNIQEYQLMSS
CYHQLLLAFRLLFAWSGFSQHENSNLLRSALQVLADRLKPGETEFLPLEE
LISESFQYLLNFQASIPSFQCAFILTQVLMAISEKPMTGWKREKMASLAK
QFLCQSWMKPGGDREKGSHFNSALHTLLCVYLEHTDNILKAIEEISSVGV
PELINSAKDGCSSTYPTLSRQTFPVFFRVMMAQLESSVKSIPAGKPSDSG
EVQLEKLLKWNIAVRNFHILINLVKVFDSRPVLSICLKYGRLFVEAFLKL
AMPLLDHSFKKHRDDVQSLLKTLQLSTRQLHHMCGHSKIHQDLGLTNHVP
LLKKSLEQFVYRVKAMLAFNHCQEAFWVGVLKNRDLQGEEILSQASAAPE
EDSAEGSEEDTADSAAEEPDGTDSDSGGAGR*

Antibiotic

Amp

Comments

chicken fancI: NM_001114851.1 GI:168803993 with silent changes to kill internal BamHI sites.

chicken fancd2: NM_001039261.2 GI:452404585 with silent changes:
t823a, c824g, t882c, g1182a, c1284t, a1593g, a1809g, g2559a, t3309c, c3663t, t3735c, t3924c, a4188g.

N.B. Propagate at low temperature.

100042 R 1000 1001 1047 19007

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