FAN1

This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
KIAA1018; KMIN; MTMR15; RP11-540B6.6
Plasmid
pcDNA5 FRT/TO.GFP.Puro.DU FAN1 (MTMR15) C44A C47A S126A F129A
Parent Plasmid
pcDNA5D FRT/TO GFP puro
Expressed
GFP-FAN1 (MTMR15) C44A C47A S126A F129A
DU Number
DU49055
Genbank
NM_014967.4 GI:226246522 with t3015c (silent)
Tag
GFP
Species
Human
Sequence of Insert
AGATCTATGATGTCAGAAGGGAAACCTCCTGACAAAAAAAGGCCTCGTAGAAGCTTATCAATCAGCAAGAATAAGAAAAAAGCATCTAATTCTATTATTTCGTGTTTTAACAATGCACCACCTGCTAAACTTGCCGCACCCGTTGCAAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGTTCAAGTGGATCCAGGGCAGGTTGGCTTAATAAATTCAAATGTGTCTATGGTAGATTTAACCAGTGTTACCTTAGAAGATGTAACACCTAAGAAGTCACCACCACCAAAGACAAATTTAACCCCTGGCCAAAGTGATTCAGCAAAAAGGGAAGTAAAGCAGAAGATCGCTCCCTACGCTAAAAGTAATGATGTGGTGTGCAAAAATCAAGATGAGCTGAGAAATCGTAGTGTGAAAGTCATTTGTTTGGGAAGCCTAGCATCTAAATTGTCCAGAAAATACGTAAAGGCTAAAAAATCAATAGATAAGGATGAAGAATTTGCCGGTTCTAGTCCACAGAGTTCCAAATCCACAGTTGTTAAGAGCCTGATTGATAACTCTTCAGAAATTGAGGACGAGGATCAAATTTTGGAGAACAGTTCTCAAAAAGAAAACGTGTTTAAATGTGATTCTCTAAAGGAAGAGTGCATTCCTGAACATATGGTAAGAGGAAGTAAAATAATGGAAGCCGAAAGCCAAAAGGCTACCCGGGAATGTGAGAAATCAGCCCTCACCCCTGGATTCTCAGATAATGCGATCATGTTATTCTCACCAGATTTCACTCTTAGGAATACATTAAAGTCTACTTCAGAAGACAGTCTTGTAAAGCAAGAGTGTATCAAAGAAGTGGTTGAAAAACGTGAGGCATGTCATTGTGAAGAAGTAAAAATGACTGTTGCTTCAGAAGCTAAAATACAGCTGTCAGATTCAGAGGCAAAATCTCATAGTTCTGCAGATGATGCTTCTGCATGGAGTAACATCCAAGAGGCTCCTCTGCAGGATGACAGTTGCTTAAACAATGATATCCCTCACAGCATTCCTTTGGAGCAGGGGTCAAGCTGCAATGGTCCTGGTCAAACAACCGGTCATCCTTACTACCTTCGGAGTTTCCTTGTGGTGCTGAAAACCGTACTTGAGAATGAAGATGATATGTTGCTCTTTGATGAGCAGGAGAAGGGAATTGTAACTAAATTTTATCAGTTATCAGCTACTGGTCAGAAGTTATATGTAAGGCTCTTTCAACGTAAATTAAGCTGGATTAAGATGACCAAATTAGAGTATGAAGAGATTGCCTTAGACTTAACACCTGTGATTGAAGAATTGACGAATGCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGAAGTGCTTGAACTCCTTTCTGCTCCTGAACTAAAATCCCTAGCCAAGACCTTCCACTTGGTGAATCCCAATGGACAGAAACAGCAGCTGGTGGACGCCTTTCTCAAATTGGCCAAACAGCGTTCAGTCTGCACTTGGGGCAAGAATAAGCCTGGAATTGGTGCAGTGATTTTAAAAAGAGCCAAAGCCTTGGCTGGACAGTCAGTACGAATCTGTAAAGGCCCCAGGGCTGTGTTTTCCCGCATCTTGCTACTGTTTTCGTTGACCGACTCAATGGAAGATGAAGACGCCGCTTGTGGAGGTCAGGGACAGCTTTCAACAGTCCTGTTGGTCAACCTCGGCCGAATGGAGTTTCCTAGTTACACCATCAATCGGAAAACCCACATCTTCCAAGACAGAGATGATCTTATCAGATATGCAGCAGCCACGCACATGCTGAGTGACATTTCTTCCGCAATGGCCAATGGGAACTGGGAAGAAGCTAAGGAGCTCGCTCAGTGTGCAAAAAGGGATTGGAACAGACTGAAAAACCACCCTTCTCTGAGATGCCACGAAGATTTACCACTCTTCCTGCGGTGTTTCACTGTTGGGTGGATTTATACAAGGATTTTGTCTCGGTTTGTGGAAATACTGCAGAGACTTCACATGTATGAGGAAGCCGTCAGAGAACTTGAAAGCCTTTTGTCTCAGAGAATTTATTGTCCTGACAGCAGAGGCCGATGGTGGGATCGACTGGCCCTTAATTTACACCAGCACTTGAAGCGCCTGGAACCGACTATCAAGTGCATCACAGAGGGGCTGGCGGATCCGGAAGTCAGAACGGGACACCGCCTTTCACTGTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGTAAAAAGTTCAAGCACCTCTTCCAGCAGCTCCCAGAAATGGCTGTGCAAGATGTGAAACACGTGACCATCACAGGCAGGCTGTGCCCACAGCGTGGGATGTGCAAGTCTGTGTTTGTGATGGAGGCCGGGGAGGCCGCTGACCCCACCACGGTCCTGTGCTCTGTGGAGGAGCTGGCACTGGCCCATTACAGACGCAGCGGTTTTGACCAGGGGATTCATGGCGAAGGGTCCACCTTCAGCACCCTGTATGGCCTCCTCCTGTGGGACATCATCTTCATGGATGGGATTCCGGATGTCTTCAGAAACGCCTGTCAGGCATTCCCCCTGGACTTGTGCACAGACAGCTTCTTCACAAGCAGACGCCCAGCCCTTGAGGCCAGGCTGCAGCTGATTCATGATGCCCCCGAGGAGAGCCTGCGGGCCTGGGTGGCAGCCACGTGGCATGAGCAGGAAGGCAGAGTGGCTTCCCTTGTCAGCTGGGATCGCTTCACGTCTCTTCAGCAAGCTCAGGATCTTGTCTCCTGCCTGGGGGGCCCTGTGCTCAGTGGTGTGTGCAGGCACCTGGCTGCTGACTTTCGACACTGTCGAGGGGGCCTCCCCGACCTGGTGGTGTGGAACTCCCAGAGCCGTCACTTTAAGCTGGTGGAAGTTAAAGGCCCCAATGATCGTCTTTCACATAAGCAGATGATCTGGCTGGCTGAACTGCAGAAGCTGGGGGCTGAAGTAGAAGTCTGCCACGTGGTTGCAGTTGGAGCTAAGAGCCAAAGCCTTAGCTAAGCGGCCGC

Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMMSEGK
PPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLAAPVASKMVPRYDL
NRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPPK
TNLTPGQSDSAKREVKQKIAPYAKSNDVVCKNQDELRNRSVKVICLGSLA
SKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQIL
ENSSQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTPG
FSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVKM
TVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSIP
LEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTK
FYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFL
QTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQ
RSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTD
SMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYA
AATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPLF
LRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRGR
WWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRES
PSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAAD
PTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGIP
DVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATW
HEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRG
GLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCHV
VAVGAKSQSLS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N.B. Has Puro selection, not Hygro.
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
Download datasheet