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VAPB

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Synonyms
VAP-B , ALS8
Plasmid
pET28a-VAPB aa1-210 -TEV-HALO (C ter)
Parent Plasmid
pET28a original
Expressed
VAPB aa1-210-HALO
DU Number
DU24180
Genbank
NM_004738.3
Tag
HALO
Species
Human
Sequence of Insert
ccATGGGTCGCGGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAG
CCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCAC
CAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGA
AGACTACAGCACCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATC
GATGCAGGGGCCTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTA
TGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGTTTG
CTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCG
GAAGACCTTATGGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGA
GAATGATAAACCACATGATGTAGAAATAAATAAAATTATATCCACAACTG
CATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTG
GATGACACCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGG
TGAAGTTCAGAGGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATG
GACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAA
TTCGAGCTCCGTCGACaagcttccGAAAACCTGTATTTTCAGGGCatggc
agaaatcggtactggctttccattcgacccccattatgtggaagtcctgg
gcgagcgcatgcactacgtcgatgttggtccgcgcgatggcacccctgtg
ctgttcctgcacggtaacccgacctcctcctacgtgtggcgcaacatcat
cccgcatgttgcaccgacccatcgctgcattgctccagacctgatcggta
tgggcaaatccgacaaaccagacctgggttatttcttcgacgaccacgtc
cgcttcatggatgccttcatcgaagccctgggtctggaagaggtcgtcct
ggtcattcacgactggggctccgctctgggtttccactgggccaagcgca
atccagagcgcgtcaaaggtattgcatttatggagttcatccgccctatc
ccgacctgggacgaatggccagaatttgcccgcgagaccttccaggcctt
ccgcaccaccgacgtcggccgcaagctgatcatcgatcagaacgttttta
tcgagggtacgctgccgatgggtgtcgtccgcccgctgactgaagtcgag
atggaccattaccgcgagccgttcctgaatcctgttgaccgcgagccact
gtggcgcttcccaaacgagctgccaatcgccggtgagccagcgaacatcg
tcgcgctggtcgaagaatacatggactggctgcaccagtcccctgtcccg
aagctgctgttctggggcaccccaggcgttctgatcccaccggccgaagc
cgctcgcctggccaaaagcctgcctaactgcaaggctgtggacatcggcc
cgggtctgaatctgctgcaagaagacaacccggacctgatcggcagcgag
atcgcgcgctggctgtcgacgctcgagatttccggcTGAgcggccgc
Amino Acid Sequence
MGRGSMAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVK
TTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFA
PTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTA
SKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDG
LRMRKTVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISG*
Vector Type
Bacterial
Antibiotic
Kan
Comments
5 additional amino acids (MGRGS) on N-terminus prior to native start
100042 R 1000 1001 1047 19007
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