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Plasmid
pcDNA5-FRT/TO-GFP DCUN1D1 K149R
Parent Plasmid
pcDNA5 FRT/TO GFP
Expressed
GFP-DCUN1D1 K149R
DU Number
DU24059
Genbank
NM_020640*
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AgAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAGCATGGCCTGTTCTTATTGATGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AgAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAGCATGGCCTGTTCTTATTGATGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMNKLKS
SQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESV
KGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIA
WKFRAATQCEFSKQEFMDGMTELGCDSIEKLKAQIPKMEQELREPGRFKD
FYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKR
SIPKDTWNLLLDFSTMIADDMSNYDEEGAWPVLIDDFVEFARPQIAGTKS
TTV*AA
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMNKLKS
SQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESV
KGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIA
WKFRAATQCEFSKQEFMDGMTELGCDSIEKLKAQIPKMEQELREPGRFKD
FYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKR
SIPKDTWNLLLDFSTMIADDMSNYDEEGAWPVLIDDFVEFARPQIAGTKS
TTV*AA
Vector Type
Mammalian
Antibiotic
Amp
Comments
*carries g318a mutation (silent Ala106) compared to NCBI ref
100042 R 1000 1001 1047 19007
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