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VAPB

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Synonyms
VAP-B , ALS8
Plasmid
pET28a- VAPB aa1-210 K87D M89D -TEV-HALO (C ter)
Parent Plasmid
pET28a C-TEV Halo1
Expressed
VAPB (aa1-210 K87D M89D) -HALO
DU Number
DU23950
Genbank
NM_004738.3
Tag
HALO
Species
Human
Sequence of Insert
GGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGA
GCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGC
TTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCA
CCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGC
CTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTATGATCCCAATG
AGAAAAGTAAACACGACTTTGACGTTCAGTCTATGTTTGCTCCAACTGAC
ACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTAT
GGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAAC
CACATGATGTAGAAATAAATAAAATTATATCCACAACTGCATCAAAGACA
GAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACACCGA
AGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGA
GGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATGGACTGCGGATG
AGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAATTC
Amino Acid Sequence
MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPR
RYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHDFDVQSMFAPTDTS
DMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTET
PIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRK
TVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLGERMHY
VDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDK
PDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVK
GIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLP
MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLL
QEDNPDLIGSEIARWLSTLEISG*
Vector Type
Bacterial
Antibiotic
Kan
Comments
no N-ter 6His tag, TEV not blunt
Parental 23919
100042 R 1000 1001 1047 19007
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