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Plasmid
pet28 6HIS GFP TEV USP35 synthetic C450S
Parent Plasmid
pET28a 6His GFP TEV
Expressed
His6-GFP-TEV-USP35 C450S
DU Number
DU46114
Genbank
Q9P2H5
Tag
GFP
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGTCAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGCCGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTCTACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCTGCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCGCGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCCCGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCCTGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCGTGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTGGCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTGCCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCGAGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTCCTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAAAGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCGCCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTTGTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGACTGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATATTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTCTAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGTACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTCCCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCGGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGTTCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATCAAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCAGGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGCTCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAACACATCCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTTCAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGACCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCCATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGCTGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAGTCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTCTGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCTGCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTCGCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGATGCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTGCACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATCACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAAGGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAGAAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAAAGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGAAGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGGAAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCCGGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGTTAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACTGCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGCCAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCTGCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGCTGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGCAGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTGCTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCGATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTGTCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAAGGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCGAGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAGGACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCAGGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACATCTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGCTCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAGACTGGTCTTCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLE
NLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALL
ALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGG
AGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACA
QVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQA
QQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPL
ELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKG
LAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHE
AFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYE
PVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIG
LINLGNTSYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLE
HSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRI
CQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEA
FTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRR
QRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEE
VGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHP
DAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAE
KVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRG
QAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYL
FNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRT
EPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDED
KDEDEGSPGGCNPAGGNGGDFHRLVF*
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLE
NLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALL
ALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGG
AGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACA
QVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQA
QQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPL
ELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKG
LAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHE
AFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYE
PVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIG
LINLGNTSYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLE
HSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRI
CQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEA
FTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRR
QRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEE
VGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHP
DAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAE
KVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRG
QAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYL
FNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRT
EPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDED
KDEDEGSPGGCNPAGGNGGDFHRLVF*
Vector Type
Bacterial
Antibiotic
Kan
100042 R 1000 1001 1047 19007
Download datasheet
Parental 25159