This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pFBDM-Rbx1, KLHL3 , DAC_TEV-Cullin3
Parent Plasmid
pFBDM
Expressed
DAC-Cullin3 (insect optimised)
DU Number
DU23402
Tag
DAC
Species
Human
Sequence of Insert
View Sequence
GGATCCGAAAACCTGTATTTTCAGATGTCTAACCTGTCTAAGGGCACTGGCAGCCGTAAGGATACCAAGATGAGAATCAGAGCCTTCCCGATGACTATGGACGAAAAGTACGTGAACTCCATCTGGGACCTGCTCAAGAACGCTATCCAGGAGATCCAAAGGAAGAACAACTCCGGCTTGTCTTTCGAGGAACTGTACAGAAACGCCTACACTATGGTGCTGCACAAGCACGGCGAGAAGCTCTACACTGGCTTGCGTGAAGTGGTCACAGAGCACCTCATCAACAAGGTTCGCGAAGATGTGCTCAACTCTTTGAACAACAACTTCCTGCAGACTCTCAACCAGGCTTGGAACGACCACCAGACAGCTATGGTCATGATCAGGGATATCCTGATGTACATGGACAGAGTCTACGTTCAGCAAAACAACGTGGAGAACGTCTACAACCTCGGATTGATCATCTTCCGTGACCAGGTTGTGCGCTACGGTTGCATCCGTGATCACCTCCGCCAAACCTTGCTGGACATGATCGCTCGTGAACGCAAGGGAGAGGTCGTTGATAGGGGTGCTATCAGAAACGCCTGTCAGATGCTGATGATCCTGGGTCTCGAAGGCAGGAGCGTCTACGAGGAAGACTTCGAGGCTCCCTTCCTCGAAATGTCGGCCGAGTTCTTCCAGATGGAATCCCAAAAGTTCTTGGCTGAGAACAGCGCCTCAGTCTACATCAAGAAGGTTGAAGCCCGTATCAACGAGGAAATCGAGCGCGTTATGCACTGCCTGGACAAGTCAACCGAGGAACCTATCGTCAAGGTGGTCGAGCGCGAACTCATCTCCAAGCACATGAAGACCATCGTCGAGATGGAAAACTCTGGTCTGGTTCACATGCTCAAGAACGGCAAGACTGAGGACCTGGGATGTATGTACAAGCTCTTCTCCAGGGTGCCCAACGGCCTGAAGACTATGTGCGAATGTATGTCCTCTTACCTCAGAGAGCAGGGAAAGGCTTTGGTTTCTGAGGAAGGCGAGGGAAAGAACCCTGTGGACTACATCCAAGGCCTCTTGGATTTGAAGTCTAGGTTCGACCGTTTCCTGCTGGAGTCCTTCAACAACGATAGGCTGTTCAAGCAGACCATCGCCGGAGACTTCGAGTACTTCTTGAACCTGAACTCCCGTTCCCCTGAATACCTCAGCTTGTTCATCGACGATAAGCTGAAGAAGGGAGTGAAGGGTCTCACCGAGCAAGAAGTCGAGACTATCTTGGATAAGGCTATGGTCCTGTTCCGTTTCATGCAGGAAAAGGACGTTTTCGAGCGCTACTACAAGCAACACCTGGCCCGTCGCTTGCTGACTAACAAGTCTGTGAGCGACGATAGCGAAAAGAACATGATCTCAAAGTTGAAGACCGAGTGCGGTTGTCAGTTCACTTCTAAGCTGGAAGGCATGTTCCGTGATATGTCAATCTCGAACACCACTATGGACGAGTTCCGCCAGCACCTGCAAGCTACCGGTGTGTCACTCGGTGGCGTGGACCTGACTGTGCGTGTCCTGACAACCGGCTACTGGCCCACTCAGTCGGCTACACCTAAGTGCAACATCCCCCCTGCTCCACGTCACGCCTTCGAAATCTTCAGGAGATTCTACTTGGCCAAGCACAGCGGACGCCAGCTGACACTCCAACACCACATGGGTTCAGCTGATCTGAACGCCACCTTCTACGGCCCGGTGAAGAAGGAAGACGGCTCCGAGGTTGGAGTGGGAGGTGCTCAGGTGACCGGTTCAAACACTCGCAAGCACATCCTGCAGGTCTCGACATTCCAAATGACCATCTTGATGCTGTTCAACAACAGGGAGAAGTACACATTCGAGGAAATCCAGCAAGAAACCGACATCCCAGAACGTGAGCTGGTGCGTGCTCTGCAGTCGCTGGCTTGCGGAAAGCCAACTCAACGCGTCTTGACAAAGGAGCCGAAGTCCAAGGAAATCGAGAACGGTCACATCTTCACAGTGAACGATCAGTTCACCTCTAAGCTGCACCGTGTTAAGATCCAGACTGTGGCTGCCAAGCAAGGCGAGAGCGACCCCGAAAGGAAGGAGACAAGACAGAAGGTGGACGATGACCGCAAGCACGAAATCGAGGCTGCCATCGTCAGGATCATGAAGAGCAGAAAGAAGATGCAACACAACGTCCTCGTTGCTGAGGTCACCCAGCAACTCAAGGCCAGGTTCTTGCCATCACCGGTTGTGATCAAGAAGAGAATCGAGGGTCTGATCGAGAGAGAATACTTGGCTAGGACTCCAGAGGACAGAAAGGTTTACACTTACGTTGCTTAAaagctt
Amino Acid Sequence
View Sequence
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*KL
Vector Type
Baculovirus
Antibiotic
Amp
Comments
blunt TEV
100042 R 1000 1001 1047 19007
Download datasheet