This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
x
Plasmid
pTYB-ISG15-Intein-6His
Parent Plasmid
pTYB
Expressed
ISG15-Intein-6His
DU Number
DU20800
Genbank
NP_005092
Tag
6His
Species
Human
Sequence of Insert
View Sequence
CATATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATTCCA
GGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGATCA
CCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCACCCG
AGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGG
CCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGA
ACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTGCGG
CTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGG
TGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGG
ACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTC
ATGAATCTGCGCCTGCGGGGAGGCTGCTTTGCCAAGGGTACC
GGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGATCA
CCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCACCCG
AGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGG
CCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGA
ACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTGCGG
CTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGG
TGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGG
ACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTC
ATGAATCTGCGCCTGCGGGGAGGCTGCTTTGCCAAGGGTACC
Amino Acid Sequence
View Sequence
MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHP
SGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLNILVRNNKGRSSTYEVR
LTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVF
MNLRLRGGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPR
GRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRL
SRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANEL
VESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDY
MQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEY
AEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVG
LGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTI
HTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVTKHKISYAIYMSGGDVLL
NVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDH
QFLLGSQVVVHACGGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKT
YKCLQPHTSLAGWEPSNVPALWQLQHHHHHH*
SGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLNILVRNNKGRSSTYEVR
LTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVF
MNLRLRGGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPR
GRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRL
SRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANEL
VESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDY
MQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEY
AEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVG
LGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTI
HTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVTKHKISYAIYMSGGDVLL
NVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDH
QFLLGSQVVVHACGGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKT
YKCLQPHTSLAGWEPSNVPALWQLQHHHHHH*
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Sce VMA intein
Parental Plasmid pYTB2 (DU32127)
Estimated MW of expressed protein : 75515 Daltons