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Plasmid
pTRE-Tight-SV5-DCUN1D1 D211A,A235R,D241A
Parent Plasmid
pTRE-Tight
Expressed
SV5-DCUN1D1 D211A,A235R,D241A
DU Number
DU20581
Genbank
NM_020640*
Tag
SV5
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCACCATGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTCAAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCTGAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTTAGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTGGAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCCAGCATTAGTGTGTTGATTATTGCGTGGAAGTTCAGAGCAGCAACACAGTGCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTGACAGCATA
GAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTGAAAGAACC
AGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATC
CAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACTGGAACTTA
GTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAATTTTTGTT
GGAACATCATAAACGATCAATACCAAAAGCCACTTGGAATCTTCTTTTAG
ACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATGAAGAAGGA
AGATGGCCTGTTCTTATTGCTGACTTTGTGGAATTTGCACGCCCTCAAAT
TGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
GAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTGAAAGAACC
AGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATC
CAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACTGGAACTTA
GTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAATTTTTGTT
GGAACATCATAAACGATCAATACCAAAAGCCACTTGGAATCTTCTTTTAG
ACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATGAAGAAGGA
AGATGGCCTGTTCTTATTGCTGACTTTGTGGAATTTGCACGCCCTCAAAT
TGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MGKPIPNPLLGLDSTMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDW
KLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDG
IQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIE
KLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLV
LNGRFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGR
WPVLIADFVEFARPQIAGTKSTTV*AA
KLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDG
IQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIE
KLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLV
LNGRFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGR
WPVLIADFVEFARPQIAGTKSTTV*AA
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
a632c (confers D211A), g703a/c704g (confers A235R) and a722c (confers D241A).
Mammalian response plasmid for expression of SV5-DCUN1D1 D211A,A235R,D241A mutant (for use with Clontech Advanced Tet-ON system)
Estimated MW of expressed protein : 31528 Daltons