VAPB | Ubiquitylation Resource

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Plasmid

pCMV5-FLAG VAPB K3R

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG-VAPB K3R

DU Number

DU20133

Genbank

NM_004738.3

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCGAGGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGA
GCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGC
TTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCA
CCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGC
CTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTATGATCCCAATG
AGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGTTTGCTCCAACTGAC
ACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTAT
GGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAAC
CACATGATGTAGAAATAAATAAAATTATATCCACAACTGCATCAAAGACA
GAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACACCGA
AGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGA
GGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATGGACTGCGGATG
AGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCATTAGCCCCAACTGG
GAAGGAAGAAGGCCTTAGCACCCGGCTCTTGGCTCTGGTGGTTTTGTTCT
TTATCGTTGGTGTAATTATTGGGAAGATTGCCTTGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMARVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRN
VCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFM
VQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINK
IISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQ
FKEEDGLRMRKTVQSNSPISALAPTGKEEGLSTRLLALVVLFFIVGVIIG
KIAL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N-terminally FLAG-tagged VAPB K3R for mammalian expression.

Estimated MW of expressed protein : 28499 Daltons

100042 R 1000 1001 1047 19007

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