1. Home
  3. Cullin2

Cullin2

This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Plasmid
pEFIRES-P-eYFP C1-Cullin2
Parent Plasmid
pEFIRES puro eYFPC1
Expressed
eYFP-Cullin2
DU Number
DU20543
Genbank
NM_003591
Tag
YFP
Species
Human
Sequence of Insert
GGATCCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTAT
ATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAA
ATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATG
AACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTG
ATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAAT
CAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGG
TTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTA
AAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGA
GTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGT
CGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCA
ACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATA
ATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTA
CTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAA
CCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAA
ACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGC
GTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTG
TTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAG
AAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAG
CTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGT
TCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCCATGTCT
ATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCG
CTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGACACAGTA
ATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTG
GCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAG
AAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGA
AGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAAAATGAA
CTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAG
GACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAAATCATT
ACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATG
CAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAA
TTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCA
GACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAGCTGCTA
TAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCAT
GATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAGAACGCA
GCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCCGC


Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMSLKPR
VVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGER
LYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYR
YLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQ
AILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEI
FESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHP
SSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVST
GLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINT
VLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGM
TENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEA
MINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGIS
FQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWL
HYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMN
EKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTS
MQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQ
SRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA*


Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression vector for eYFP-Cullin2. EF1a promoter, IRES and puromycin selection cassette

Estimated MW of expressed protein : 114458 Daltons
100042 R 1000 1001 1047 19007
Download datasheet