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RNF220

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Plasmid
pEFIRES-P-eYFP C1-RNF220
Parent Plasmid
pEFIRES puro eYFPC1
Expressed
eYFP-RNF220
DU Number
DU20544
Genbank
NM_018150.2
Tag
YFP
Species
Human
Sequence of Insert
GGATCCATGGACTTACACCGGGCAGCCTTCAAGATGGAGAACTCATCCTA
CCTTCCCAACCCTCTGGCATCCCCAGCACTGATGGTCCTGGCATCCACGG
CTGAGGCCAGCCGTGATGCTTCCATCCCTTGTCAGCAGCCACGACCCTTT
GGTGTACCTGTCTCAGTTGACAAGGACGTGCATATTCCTTTCACCAACGG
TTCCTATACCTTTGCCTCTATGTACCATCGGCAAGGTGGGGTGCCAGGCA
CTTTTGCCAATCGTGATTTCCCCCCTTCTCTACTACACCTCCACCCTCAA
TTTGCTCCCCCAAATCTAGATTGCACCCCAATCAGTATGCTGAATCATAG
TGGTGTGGGGGCTTTCCGGCCCTTTGCCTCCACCGAGGACCGGGAGAGCT
ATCAGTCAGCCTTTACGCCGGCCAAGCGACTTAAGAACTGCCATGACACA
GAGTCTCCCCACTTGCGCTTCTCAGATGCAGATGGCAAGGAATATGACTT
TGGGACACAGCTGCCATCTAGCTCCCCCGGTTCACTAAAGGTTGATGACA
CTGGGAAGAAGATTTTTGCTGTCTCTGGCCTCATTTCTGATCGGGAAGCC
TCATCTAGCCCAGAGGATCGGAATGACAGATGTAAGAAGAAAGCAGCGGC
ATTGTTCGACAGCCAGGCCCCAATTTGCCCCATCTGCCAGGTCCTGCTGA
GGCCCAGTGAGCTGCAGGAGCATATGGAGCAGGAACTGGAGCAGCTAGCC
CAACTGCCCTCGAGCAAGAATTCCCTTCTGAAGGATGCCATGGCTCCAGG
CACCCCAAAGTCCCTCCTGTTGTCTGCTTCCATCAAGAGGGAAGGAGAGT
CTCCAACGGCATCACCCCACTCATCTGCCACCGATGACCTCCACCATTCA
GACAGATACCAGACCTTTCTGCGAGTACGAGCCAACCGGCAGACCCGACT
GAATGCTCGGATTGGGAAAATGAAACGGAGGAAGCAAGATGAAGGGCAGA
GGGAAGGCTCCTGCATGGCTGAGGATGATGCTGTGGACATCGAGCATGAG
AACAACAACCGCTTTGAGGAGTATGAGTGGTGTGGACAGAAGCGGATACG
GGCCACCACTCTCCTGGAAGGTGGCTTCCGAGGCTCTGGCTTCATCATGT
GCAGCGGCAAAGAGAACCCGGACAGTGATGCTGACTTGGATGTGGATGGG
GATGACACTCTGGAGTATGGGAAGCCACAATACACAGAGGCTGATGTCAT
CCCCTGCACAGGCGAGGAGCCTGGTGAAGCCAAGGAGAGAGAGGCACTTC
GGGGCGCAGTCCTAAATGGCGGCCCTCCCAGCACGCGCATCACACCTGAG
TTCTCTAAATGGGCCAGTGATGAGATGCCATCCACCAGCAATGGTGAAAG
CAGCAAGCAGGAGGCCATGCAGAAGACCTGCAAGAACAGCGACATCGAGA
AAATCACCGAAGATTCAGCTGTGACCACGTTTGAGGCTCTGAAGGCTCGG
GTCAGAGAACTTGAACGGCAGCTATCTCGTGGGGACCGTTACAAATGCCT
CATCTGCATGGACTCGTACTCGATGCCCCTAACGTCCATCCAGTGTTGGC
ACGTGCACTGCGAGGAGTGCTGGCTGCGGACCCTGGGTGCCAAGAAGCTC
TGCCCTCAGTGCAACACGATCACAGCGCCCGGAGACCTGCGGAGGATCTA
CTTGTGAGCGGCCGC


Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMDLHRA
AFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDK
DVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDC
TPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFS
DADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRN
DRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPSSKNS
LLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLR
VRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEY
EWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGK
PQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDE
MPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQL
SRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTIT
APGDLRRIYL*



Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression vector for eYFP-Rnf220. EF1a promoter, IRES and puromycin selection cassette

Estimated MW of expressed protein : 90240 Daltons
100042 R 1000 1001 1047 19007
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