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USP3

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Plasmid
pET28-DAC-USP3
Parent Plasmid
pET28a-DAC (SC20568)
Expressed
DAC-USP3
DU Number
DU20633
Tag
DAC
Sequence of Insert
GGATCCATGGAGTGTCCACACCTGAGCTCCAGCGTCTGCATTGCTCCGGACTCAGCCAAGTTCCCCAACGGCTCCCCGTCGTCCTGGTGCTGCAGCGTGTGCCGGTCCAACAAAAGCCCTTGGGTCTGTTTGACTTGTTCAAGTGTCCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAAGTACCTTTAACCAACCATAAGAAATCAGAAAAGCAAGATAAAGTTCAGCACACAGTATGTATGGATTGCAGTAGCTACAGTACATACTGTTATCGCTGTGATGATTTTGTGGTTAATGACACCAAGCTGGGACTGGTACAGAAAGTCAGAGAACACTTACAGAACTTGGAAAACTCAGCTTTCACAGCTGACAGGCATAAGAAAAGAAAACTTTTGGAAAACTCAACACTAAACAGCAAGTTATTAAAAGTAAATGGAAGCACCACTGCCATTTGTGCCACAGGCCTTCGGAATTTGGGGAACACATGTTTCATGAATGCCATCCTTCAGTCACTCAGTAACATTGAGCAGTTTTGCTGTTATTTCAAAGAACTGCCCGCCGTGGAGTTAAGGAATGGGAAAACAGCAGGAAGGCGGACATACCACACCAGGAGCCAAGGGGATAACAATGTGTCTTTGGTAGAAGAGTTTAGAAAGACACTCTGTGCTTTATGGCAAGGCAGCCAGACTGCATTTAGCCCAGAGTCCTTATTTTATGTTGTTTGGAAGATTATGCCAAACTTTAGGGGCTATCAACAGCAGGACGCCCATGAATTCATGCGCTACCTTTTGGACCACCTACACTTGGAACTTCAGGGCGGTTTCAACGGTGTTTCCCGCTCAGCAATTCTGCAGGAGAATTCTACTCTGTCTGCAAGTAACAAGTGTTGCATAAATGGAGCATCTACTGTTGTCACGGCTATATTCGGAGGCATTCTCCAAAATGAGGTTAACTGCCTCATATGTGGGACAGAATCTAGAAAGTTTGATCCATTCCTAGACCTTTCATTAGATATTCCAAGTCAGTTCAGAAGTAAGCGCTCTAAGAATCAAGAAAATGGACCAGTTTGTTCGTTACGAGATTGTCTTCGCAGTTTTACCGACTTAGAAGAACTTGATGAGACAGAGTTATATATGTGCCATAAATGCAAAAAGAAACAAAAGTCCACAAAAAAGTTTTGGATTCAAAAACTACCCAAGGTGCTATGCTTACATTTGAAAAGATTTCATTGGACAGCATATTTAAGAAATAAAGTTGATACATACGTAGAATTTCCACTGAGAGGCCTAGACATGAAATGCTACTTACTAGAGCCTGAGAACAGTGGCCCGGAGAGCTGCCTGTATGACCTCGCCGCTGTGGTGGTGCACCATGGTTCCGGGGTTGGTTCTGGACATTACACAGCATACGCAACTCACGAAGGCCGCTGGTTCCACTTCAATGACAGTACTGTAACACTGACTGACGAGGAGACTGTGGTGAAGGCGAAGGCCTACATCCTTTTCTACGTGGAACACCAGGCCAAAGCTGGATCGGATAAACTTTAAGCGGCCGC
Amino Acid Sequence
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMECPHLSSSVCIAPDSAKFPNGSPSSWCC
SVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDK
VQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTAD
RHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSN
IEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCAL
WQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGG
FNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGT
ESRKFDPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDE
TELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVE
FPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHE
GRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL*

Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression vector for N-terminally DAC tagged USP13 (contains thrombin cleavage site)

Estimated MW of expressed protein : 88269 Daltons
100042 R 1000 1001 1047 19007
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