This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pETDuet-6His_TEV-Rbx1, GST_TEV-Cullin3 (codon optimised bacterial)
Parent Plasmid
pETDuet1
Expressed
6His-Rbx1
DU Number
DU23253
Tag
6His
Sequence of Insert
View Sequence
agatctATGCATCATCACCATCACCATGAAAACCTGTATTTTCAGATGGCAGCAGCAATGGATGTTGATACGCCGTCGGGTACGAACTCGGGTGCGGGTAAAAAACGCTTTGAAGTGAAAAAATGGAATGCGGTTGCACTGTGGGCTTGGGATATCGTGGTTGACAACTGCGCAATTTGTCGTAATCATATCATGGATCTGTGCATTGAATGTCAGGCCAACCAAGCGAGCGCCACCTCTGAAGAATGCACGGTCGCGTGGGGCGTGTGTAATCACGCCTTTCATTTCCACTGCATTAGTCGCTGGCTGAAAACCCGTCAGGTGTGCCCGCTGGATAACCGCGAATGGGAATTTCAAAAATATGGTCACTAAGGtacc
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQMAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
both genes optimised for expression in E. coli, both genes preceded by Blunt TEV (ENLYFQ straight into Met)
100042 R 1000 1001 1047 19007
Download datasheet