USP5 | Ubiquitylation Resource

Plasmid

pET156P-N-terminal YFP Topaz FRET Acceptor

Parent Plasmid

pET15b 6His 3C

Expressed

6His-YFP Topaz ZnF UBP

DU Number

DU20519

Genbank

P45974 U47927.1

Tag

YFP

Species

Human

Sequence of Insert

View Sequence

GGATCCAGCGAATTTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCAC
CGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACA
AGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTG
ACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC
CCTCGTGACCACCTTCGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCG
ACCACATGAGGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC
GTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCG
CGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGA
AGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAG
TACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAA
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG
TGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCC
GTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAA
AGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCG
CCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGCTCGAAACAGAC
CAGACTAGTTTGTACAAAAAAGCAGGCACAGTCGACAAGCAGGAGGTGCA
GGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCA
AGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCC
AAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCAT
CCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTG
TGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACC
ATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGT
CCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGC
TGAAGATGCAGAAGACAGACAAGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSSEFATMVSKGEELFTGVVPILVELDGD
VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCF
ARYPDHMRQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNI
EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKLETDQTSLYKKAGTVDKQEVQAWDGEVRQVSKH
AFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGG
NNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHF
GIDMLKMQKTDK*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression construct to express 6HIS-YFP Topaz-ZnF UBP (Ubiquitin Specific Protease Zinc Finger USP5) FRET Acceptor (contains preScission protease site)

Estimated MW of expressed protein : 46292 Daltons

100042 R 1000 1001 1047 19007