OTUB1

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Plasmid
pcDNA5-FRT/TO-GFP OTUB1
Parent Plasmid
pcDNA5 FRT/TO GFP
Expressed
GFP-OTUB1
DU Number
DU20859
Genbank
NM_017670
Tag
GFP
Species
Human
Sequence of Insert
GGATCCATGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGG
CAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGG
CTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTG
TCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGA
CAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACA
TCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTC
TCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAA
GGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTG
AATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAG
GTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCA
GAGCACCTCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCT
ACCTGCAGCGCGAGAGCAAGTTCTTCGAGCACTTCATCGAGGGTGGACGG
ACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAG
CGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCC
AGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATC
TTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACA
CTACGATATCCTCTACAAATAGGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAAEEP
QQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSV
LYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLD
DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVA
DLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQ
EVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPK
VYLLYRPGHYDILYK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GFP-tagged OTUB1 for mammalian expression Flp-In vector

Estimated MW of expressed protein : 58609 Daltons
100042 R 1000 1001 1047 19007
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