USP5 | Ubiquitylation Resource

Plasmid

pET156P-C-terminal YFP Topaz FRET Acceptor

Parent Plasmid

pET15b 6His 3C

Expressed

6His-ZnF UBP-YFP Topaz

DU Number

DU20520

Genbank

P45974 U47927.1

Tag

YFP

Species

Human

Sequence of Insert

View Sequence

GGATCCAGCGAATTTGCCACCAAGCAGGAGGTGCAGGCATGGGATGGGGA
AGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGACAACC
CTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGA
GAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACG
CTACTTCGATGGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAG
AGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGA
GCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCT
GGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGA
CAGACAAGCTCGAAACAGACCAGACTAGTTTGTACAAAAAAGCAGGCACA
GTCGACATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTT
CGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAGGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAA
GCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
TCGGCATGGACGAGCTGTACAAGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSSEFATKQEVQAWDGEVRQVSKHAFSLK
QLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAV
EHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDML
KMQKTDKLETDQTSLYKKAGTVDMVSKGEELFTGVVPILVELDGDVNGHK
FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPD
HMRQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK
GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSV
QLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTA
AGITLGMDELYK*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression construct to express 6HIS- ZnF UBP (Ubiquitin Specific Protease Zinc Finger USP5) -YFP Topaz- FRET Acceptor (contains preScission protease site)

Estimated MW of expressed protein : 46292 Daltons

100042 R 1000 1001 1047 19007