RNF4 | Ubiquitylation Resource

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Plasmid

pcDNA3.1-FLAG-RNF4 SIM_4 mutated

Parent Plasmid

pcDNA3.1

Expressed

FLAG-RNF4 SIM_4 mutated

DU Number

DU21502

Tag

FLAG

Species

Rat

Sequence of Insert

View Sequence

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGATGAGTACAAGAAATCCTCAAAGAAAGCGGCGTGGTGGAGCTGTGAATTCTAGACAAACCCAGAAGCGAACTCGGGAAACAACTTCAACCCCTGAGATTTCCTTGGAAGCAGAACCCATAGAACTTGTGGAAACCGTTGGAGATGAAATCGTGGACCTCACCTGTGAATCTTTAGAGCCTGTGGTTGTGGACCTGACTCACAATGACTCTGCTGCAGCTGCGGAAGAAAGGAGAAGGCCAAGGAGAAATGGGAGGAGGTTGCGCCAAGACCATGCTGACAGCTGTGTGGTGAGCAGTGATGATGAAGAACTGTCTAAGGACAAAGATGTATACGTGACTACCCATACTCCTCGAAGCACCAAGGATGAAGGAACTACTGGACTTAGGCCCTCGGGCACCGTCAGTTGTCCGATCTGCATGGATGGATACTCTGAGATTGTGCAGAATGGGCGTCTCATTGTTTCTACAGAATGTGGCCACGTCTTCTGTAGCCAGTGCCTCCGTGATTCCCTTAAGAATGCTAACACTTGCCCAACTTGTAGGAAAAAGATCAACCATAAACGGTACCATCCCATTTATATATGATAA

Amino Acid Sequence

View Sequence

MDYKDDDDKMSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIE
LVETVGDEIVDLTCESLEPVVVDLTHNDSAAAAEERRRPRRNGRRLRQDH
ADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMD
GYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHP
IYI**

Vector Type

Mammalian

Antibiotic

Amp; Kan

Comments

mammalian expression plasmid for FLAG-RNF4 SIM_4 mutated

Estimated MW of expressed protein : 22897 Daltons

100042 R 1000 1001 1047 19007

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