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Cullin2

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Plasmid
pCMV Cullin2-FLAG
Parent Plasmid
pCMV5 C-FLAG
Expressed
Cullin2-FLAG
DU Number
DU20495
Genbank
NM_003591
Tag
FLAG
Species
Human
Sequence of Insert
GGATCCGCCACCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTA
AAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGA
GTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGT
CGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCA
ACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATA
ATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTA
CTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAA
CCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAA
ACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGC
GTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTG
TTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAG
AAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAG
CTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGT
TCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCCATGTCT
ATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCG
CTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGACACAGTA
ATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTG
GCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAG
AAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGA
AGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAAAATGAA
CTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAG
GACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAAATCATT
ACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATG
CAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAA
TTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCA
GACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAGCTGCTA
TAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCAT
GATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAGAACGCA
GCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGGCGGCCGC
Amino Acid Sequence
MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYP
EPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADY
MDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKL
MVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPL
KFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRC
RKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVL
LRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKF
VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLK
KSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMS
MDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTV
IDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSG
RKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQ
DSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTK
FKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI
QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVAAAADY
KDDDDK**
Vector Type
Mammalian
Antibiotic
Amp
Comments
C-terminally FLAG tagged Cullin2 for mammalian expression

Estimated MW of expressed protein : 88192 Daltons
100042 R 1000 1001 1047 19007
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