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Plasmid
pCMV5-FLAG-1 Cullin2 K689R No ATG
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG Cul2 K689R No ATG
DU Number
DU20291
Genbank
NM_003591
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAA
CAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAA
GAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCC
TATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTT
GGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAAC
AAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCA
GACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAA
GAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGG
AAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCG
AGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCC
ATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTC
CCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAAC
AGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACT
GCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATT
CGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGA
ATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAAT
GTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTAC
GTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCT
GCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTC
AGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGT
AAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTAT
GAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTA
AGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTA
CTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCT
CACGAGCTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTC
AAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCC
ATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTG
TGGTTATGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTG
TCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGAC
ACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGG
TGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGG
AATTAGAAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTC
AGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAA
AATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAA
TGGCAGTTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAG
CTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAA
ATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATA
TTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGA
ACAAAATTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAAT
GGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAG
CTGCTATAGTTCGTATCATGAGAGCACGAAAAGTGCTTCGGCACAATGCC
CTTATTCAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTAT
CAGCATGATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAG
AACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCC
GC
CAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAA
GAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCC
TATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTT
GGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAAC
AAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCA
GACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAA
GAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGG
AAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCG
AGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCC
ATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTC
CCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAAC
AGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACT
GCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATT
CGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGA
ATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAAT
GTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTAC
GTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCT
GCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTC
AGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGT
AAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTAT
GAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTA
AGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTA
CTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCT
CACGAGCTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTC
AAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCC
ATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTG
TGGTTATGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTG
TCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGAC
ACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGG
TGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGG
AATTAGAAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTC
AGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAA
AATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAA
TGGCAGTTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAG
CTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAA
ATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATA
TTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGA
ACAAAATTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAAT
GGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAG
CTGCTATAGTTCGTATCATGAGAGCACGAAAAGTGCTTCGGCACAATGCC
CTTATTCAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTAT
CAGCATGATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAG
AACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCC
GC
Amino Acid Sequence
View Sequence
DYKDDDDKGSSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMRARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA
Vector Type
Mammalian
Comments
Estimated MW of expressed protein : 88020 Daltons
100042 R 1000 1001 1047 19007
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