USP49 | Ubiquitylation Resource

Plasmid

pGEX6P-USP49 L240-end (cat domain)

Parent Plasmid

pGEX6P3

Expressed

GST-USP49 240-end (cat domain)

DU Number

DU36250

Genbank

NM_018561.3 GI:34147686

Tag

GST

Species

Human

Sequence of Insert

View Sequence

AGATCTCTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGG
CCTGCGCAACCTGGGCAACACCTGCTACATGAACTCCATCCTCCAGGTGC
TCAGCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCC
AAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTC
TGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACA
GGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCC
ATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCA
CATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCG
GGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGC
CTGATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCT
CTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCA
CACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTC
TTAAAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCAC
ATGTATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACC
TATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTC
CCTTTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCAC
AGAGACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACA
GCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGA
AAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAA
AAGATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATG
TCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATG
CTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGT
CATGCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCT
ACAACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACG
GAAAGGGGTTGAAGATCT

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLKLRRQPAMAPGVTGLRNL
GNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPT
NSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLC
RELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELL
HKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISC
NYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEA
LEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRW
SGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHG
KGFGSGHYTAYCYNTEGGACALLCGVGDTERG*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial construct to express GST N terminal fusion

Estimated MW of expressed protein : 72783 Daltons

100042 R 1000 1001 1047 19007