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Plasmid
pET156P USP4
Parent Plasmid
pET15b 6His 3C
Expressed
6His-USP4
DU Number
DU14350
Genbank
NM_003363
Tag
6His
Sequence of Insert
View Sequence
GGATCCATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGAC
TCAGAAGTCCGAGCTTGGACCCTTAATGAGGACCACACTCCAACGCGGGG
CGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAAGTAT
GTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATT
TCCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGTCAGA
CCTTGAAAGAACACTTAATTGATGAATTGGACTATGTATTGGTCCCTACC
GAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCA
ACCCATCGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCA
AAGTCGAGGTGTATTTGCTGGAACTGAAGCTCTGTGAGAACAGTGACCCC
ACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAACCAT
CGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACAC
GGCTCTGGAACAAATACATGAGCAACACCTACGAGCAGTTGAGCAAGCTA
GACAACACTGTCCAGGATGCTGGGCTATACCAGGGTCAGGTGCTAGTAAT
TGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAA
AATCAAGCACTGCGCCTAGCAGAAATTTTACTACCTCTCCAAAATCATCA
GCAAGTCCCTATTCCTCAGTGTCTGCCTCTCTCATTGCAAATGGTGATAG
CACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGATCTG
GCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAA
CCTGGGCTCTGTGGACTTGGAAACCTGGGAAACACCTGCTTCATGAACTC
CGCTTTGCAGTGTTTGAGCAACACTGCACCACTGACTGACTACTTTCTCA
AAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAA
GGGGAAATTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGG
AAGGGACGCCCATGTGGCACCTCGCATGTTCAAAACTCAAGTAGGACGTT
TTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTGCTG
GCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAA
GCCCTACTTGGAGCTGAAGGATGCCAATGGGCGGCCAGATGCGGTGGTGG
CAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATGATTCTGTGATTGTG
GATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGC
TAAGGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGC
CCTTGAAGAAAGATCGAGTTATGGAGGTTTTCCTGGTTCCTGCTGACCCT
CACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGCTGT
GTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAA
ATATGGTGGTCGCAGATGTGTATAATCACCGATTCCACAAAATTTTCCAA
ATGGATGAAGGTTTAAACCACATCATGCCTCGGGATGACATTTTCGTGTA
CGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAG
TCTACTTCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCA
GCGCTATATGGGCAGCCACTATTGCTTTCTGTCCCCAAGCACAAGTTAAC
CCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTATGTGA
AACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCC
TGCAATGGCTCCAGGAACAGCTGTGAAGGAGAAGATGAGGAAGAAATGGA
GCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACAGAAGGCAGTGGGG
AAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAA
GGCCAGCCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTA
TGGAACAGCTGACATAAATTCACTTGCAGCTGATGGAAAACTACTTAAAC
TCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCGGAGA
CTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCAT
GTTGCAGCCTCAGAAGAAGAAGAAGACCACAGTGGCCCTGAGAGACTGCA
TCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGACCCCTGGTAC
TGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATG
GTCCTTGCCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACA
GATACTGGAGGGATAAGCTCGACACAGTCGTAGAATTCCCAATCAGAGGG
CTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATGTGTA
CGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACT
ACACTGCATATGCGAAGAACAAACTGAATGGTAAATGGTATTACTTTGAT
GATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATAGTGACTAAAGCAGC
TTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTT
CACTTAGCAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCT
CAGCAGGGCTTTGGGGATGATGAGGCTTGCAGCATGGACACCAACTAAGC
GGCCGC
TCAGAAGTCCGAGCTTGGACCCTTAATGAGGACCACACTCCAACGCGGGG
CGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAAGTAT
GTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATT
TCCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGTCAGA
CCTTGAAAGAACACTTAATTGATGAATTGGACTATGTATTGGTCCCTACC
GAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCA
ACCCATCGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCA
AAGTCGAGGTGTATTTGCTGGAACTGAAGCTCTGTGAGAACAGTGACCCC
ACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAACCAT
CGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACAC
GGCTCTGGAACAAATACATGAGCAACACCTACGAGCAGTTGAGCAAGCTA
GACAACACTGTCCAGGATGCTGGGCTATACCAGGGTCAGGTGCTAGTAAT
TGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAA
AATCAAGCACTGCGCCTAGCAGAAATTTTACTACCTCTCCAAAATCATCA
GCAAGTCCCTATTCCTCAGTGTCTGCCTCTCTCATTGCAAATGGTGATAG
CACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGATCTG
GCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAA
CCTGGGCTCTGTGGACTTGGAAACCTGGGAAACACCTGCTTCATGAACTC
CGCTTTGCAGTGTTTGAGCAACACTGCACCACTGACTGACTACTTTCTCA
AAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAA
GGGGAAATTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGG
AAGGGACGCCCATGTGGCACCTCGCATGTTCAAAACTCAAGTAGGACGTT
TTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTGCTG
GCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAA
GCCCTACTTGGAGCTGAAGGATGCCAATGGGCGGCCAGATGCGGTGGTGG
CAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATGATTCTGTGATTGTG
GATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGC
TAAGGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGC
CCTTGAAGAAAGATCGAGTTATGGAGGTTTTCCTGGTTCCTGCTGACCCT
CACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGCTGT
GTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAA
ATATGGTGGTCGCAGATGTGTATAATCACCGATTCCACAAAATTTTCCAA
ATGGATGAAGGTTTAAACCACATCATGCCTCGGGATGACATTTTCGTGTA
CGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAG
TCTACTTCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCA
GCGCTATATGGGCAGCCACTATTGCTTTCTGTCCCCAAGCACAAGTTAAC
CCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTATGTGA
AACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCC
TGCAATGGCTCCAGGAACAGCTGTGAAGGAGAAGATGAGGAAGAAATGGA
GCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACAGAAGGCAGTGGGG
AAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAA
GGCCAGCCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTA
TGGAACAGCTGACATAAATTCACTTGCAGCTGATGGAAAACTACTTAAAC
TCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCGGAGA
CTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCAT
GTTGCAGCCTCAGAAGAAGAAGAAGACCACAGTGGCCCTGAGAGACTGCA
TCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGACCCCTGGTAC
TGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATG
GTCCTTGCCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACA
GATACTGGAGGGATAAGCTCGACACAGTCGTAGAATTCCCAATCAGAGGG
CTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATGTGTA
CGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACT
ACACTGCATATGCGAAGAACAAACTGAATGGTAAATGGTATTACTTTGAT
GATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATAGTGACTAAAGCAGC
TTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTT
CACTTAGCAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCT
CAGCAGGGCTTTGGGGATGATGAGGCTTGCAGCATGGACACCAACTAAGC
GGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSMAEGGGCRERPDAETQKSELGPLMRTT
LQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSD
PESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLF
VKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPA
ERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQ
TLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVS
RGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT
DYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKT
QVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRP
DAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYL
TLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSG
IAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSEC
VTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRI
SRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSET
EGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADG
KLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVA
LRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKR
FSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAM
GVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEF
YKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN*
LQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSD
PESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLF
VKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPA
ERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQ
TLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVS
RGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT
DYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKT
QVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRP
DAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYL
TLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSG
IAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSEC
VTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRI
SRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSET
EGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADG
KLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVA
LRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKR
FSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAM
GVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEF
YKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Not available - use DU23032 instead.
Estimated MW of expressed protein : 111006 Daltons