USP49 | Ubiquitylation Resource

Plasmid

pMalp2X USP49 L240-end (cat domain)

Parent Plasmid

pMALP2X

Expressed

MBP-USP49 240-end (cat domain)

DU Number

DU36251

Genbank

NM_018561.3 GI:34147686

Tag

MBP

Species

Human

Sequence of Insert

View Sequence

AGATCTCTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGG
CCTGCGCAACCTGGGCAACACCTGCTACATGAACTCCATCCTCCAGGTGC
TCAGCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCC
AAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTC
TGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACA
GGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCC
ATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCA
CATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCG
GGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGC
CTGATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCT
CTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCA
CACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTC
TTAAAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCAC
ATGTATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACC
TATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTC
CCTTTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCAC
AGAGACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACA
GCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGA
AAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAA
AAGATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATG
TCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATG
CTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGT
CATGCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCT
ACAACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACG
GAAAGGGGTTGAAGATCT

Amino Acid Sequence

View Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRISEFGSLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTERG*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial construct to express MBP N terminal fusion

Estimated MW of expressed protein : 91491 Daltons

100042 R 1000 1001 1047 19007