USP14 | Ubiquitylation Resource

Plasmid

pET156P USP14

Parent Plasmid

pET15b 6His 3C

Expressed

6His-USP14

DU Number

DU14352

Genbank

AAH03556.1

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATT
TGAAGGTGTAGAATTGAATACAGATGAACCTCCAATGGTATTCAAGGCTC
AGCTGTTTGCGTTGACTGGAGTCCAGCCTGCCAGACAGAAAGTTATGGTG
AAAGGAGGAACGCTAAAGGATGATGATTGGGGAAACATCAAAATAAAAAA
TGGAATGACTCTACTAATGATGGGGTCAGCAGATGCTCTTCCAGAAGAAC
CCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAGAAGAACAGTTAGCA
TCTGCTATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTA
CATGAATGCCACAGTTCAGTGTATTCGTTCTGTGCCTGAACTCAAAGATG
CCCTTAAAAGGTATGCAGGTGCCTTGAGAGCTTCAGGGGAAATGGCTTCA
GCGCAGTATATTACTGCAGCCCTTAGAGATTTGTTTGATTCCATGGATAA
AACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGCACATGG
CTTTCCCACAGTTTGCCGAGAAAGGTGAACAAGGACAGTATCTTCAACAG
GATGCTAATGAATGTTGGATACAAATGATGCGAGTATTGCAACAGAAATT
GGAAGCAATAGAGGATGATTCTGTTAAAGAGACAGACTCCTCATCTGCAT
CGGCAGCGACACCTTCTAAAAAGAAAAGTTTAATCGATCAGTTCTTCGGT
GTTGAGTTTGAAACTACCATGAAATGTACAGAATCTGAAGAAGAAGAAGT
CACCAAAGGAAAGGAAAATCAACTTCAGCTTAGCTGTTTTATCAATCAGG
AAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAAGAAATC
ACCAAACAGTCTCCAACGTTGCAAAGAAATGCCTTGTATATCAAATCTTC
CAAGATCAGCCGGCTGCCTGCTTACTTGACCATTCAGATGGTTCGATTTT
TTTATAAAGAGAAGGAATCTGTGAATGCCAAAGTTCTTAAGGATGTTAAA
TTTCCTCTTATGTTGGATATGTATGAACTGTGTACACCAGAACTTCAAGA
GAAAATGGTGTCTTTTCGATCCAAATTCAAGGATCTAGAAGATAAAAAAG
TGAATCAGCAGCCAAATACAAGTGACAAAAAGAGTAGTCCCCAGAAAGAA
GTTAAGTATGAACCCTTTTCTTTTGCTGATGATATTGGCTCCAATAATTG
TGGATACTATGACTTACAAGCAGTACTAACACACCAGGGAAGGTCTAGTT
CTTCAGGTCATTATGTATCATGGGTGAAAAGGAAACAAGATGAATGGATT
AAGTTTGATGATGACAAAGTCAGCATCGTAACACCAGAAGATATCTTACG
GCTTTCTGGTGGTGGAGACTGGCATATCGCTTACGTTCTACTCTATGGGC
CTCGCAGAGTTGAAATAATGGAAGAGGAAAGTGAACAGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSMPLYSVTVKWGKEKFEGVELNTDEPPM
VFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADA
LPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVP
ELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQ
FLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETD
SSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSC
FINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQ
MVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDL
EDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQ
GRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYV
LLYGPRRVEIMEEESEQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 58512 Daltons

100042 R 1000 1001 1047 19007