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Plasmid
pFB1 GST 6P-1 optineurin
Parent Plasmid
pFastbac1
Expressed
GST-Optineurin
DU Number
DU4715
Genbank
AF420371
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGTCCCA
TCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCA
CAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACC
CCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCA
GCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTG
AGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTT
GAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGA
GAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAA
GGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCG
GAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGA
GAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACT
CCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGA
GAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAG
AACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAG
ATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTC
CTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGC
ACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTA
ATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAAT
GATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAA
CCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAAC
TCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCC
CTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGA
GCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTAT
CAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTA
ACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGC
ATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACA
GGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCT
CTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAA
GCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAG
TTTACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCAT
GAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAA
TGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTC
GTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAA
AGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCA
TTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGA
TTCACGTGATGGATTGCATCATTTAAGCGGCCGCG
TCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCA
CAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACC
CCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCA
GCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTG
AGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTT
GAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGA
GAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAA
GGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCG
GAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGA
GAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACT
CCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGA
GAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAG
AACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAG
ATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTC
CTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGC
ACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTA
ATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAAT
GATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAA
CCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAAC
TCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCC
CTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGA
GCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTAT
CAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTA
ACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGC
ATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACA
GGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCT
CTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAA
GCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAG
TTTACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCAT
GAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAA
TGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTC
GTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAA
AGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCA
TTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGA
TTCACGTGATGGATTGCATCATTTAAGCGGCCGCG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLN
SSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSA
DGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTS
NRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTK
LSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESML
SEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVD
RAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQME
VYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQS
RHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQ
IHVMDCII*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLN
SSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSA
DGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTS
NRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTK
LSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESML
SEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVD
RAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQME
VYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQS
RHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQ
IHVMDCII*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Baculovirus expression with N term GST.
100042 R 1000 1001 1047 19007
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