Cullin2 | Ubiquitylation Resource

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Plasmid

pCMV5-FLAG-1 Cullin2 No ATG

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG Cul2 (No ATG)

DU Number

DU20258

Genbank

NM_003591

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

TCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACT
TTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAA
CATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCT
GAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAA
TCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTAC
TTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTAT
ATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAA
ATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATG
AACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTG
ATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAAT
CAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGG
TTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTA
AAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGA
GTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGT
CGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCA
ACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATA
ATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTA
CTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAA
CCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAA
ACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGC
GTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTG
TTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAG
AAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAG
CTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGT
TCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCCATGTCT
ATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCG
CTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGACACAGTA
ATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTG
GCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAG
AAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGA
AGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAAAATGAA
CTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAG
GACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAAATCATT
ACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATG
CAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAA
TTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCA
GACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAGCTGCTA
TAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCAT
GATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAGAACGCA
GCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGA

Amino Acid Sequence

View Sequence

DYKDDDDKGSSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFS
DIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRY
WEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIG
ELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVH
VEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVL
GRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEK
KNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFV
ESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPEL
LAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLA
KRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKF
NNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMF
ELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNN
SETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSL
NMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKA
RKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADE
YSYVA

Vector Type

Mammalian

Antibiotic

Amp

Comments

Estimated MW of expressed protein : 87992 Daltons

100042 R 1000 1001 1047 19007

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