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Plasmid
pGEX6P-1-Optineurin E50K D474N
Parent Plasmid
pGEX6P1
Expressed
GST-Optineurin E50K D474N
DU Number
DU4825
Genbank
AF420371
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCC
ATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGC
ACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTAC
CCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCAAGAACCACC
AGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTT
GAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTT
TGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATG
AGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAA
AGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGC
GGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAG
AGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAAC
TCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGG
AGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGA
GAACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCA
GATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCT
CCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTG
CACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGT
AATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAA
TGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGA
ACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAA
CTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGC
CCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAG
AGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTA
TCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATT
AACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATG
CATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGAC
AGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGC
TCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCA
AGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAA
GTTTACTGTTCTAATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCA
TGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGA
ATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGT
CGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCA
AAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTC
ATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAG
ATTCACGTGATGGATTGCATCATTTAAGCGGCCGC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCC
ATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGC
ACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTAC
CCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCAAGAACCACC
AGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTT
GAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTT
TGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATG
AGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAA
AGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGC
GGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAG
AGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAAC
TCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGG
AGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGA
GAACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCA
GATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCT
CCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTG
CACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGT
AATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAA
TGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGA
ACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAA
CTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGC
CCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAG
AGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTA
TCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATT
AACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATG
CATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGAC
AGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGC
TCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCA
AGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAA
GTTTACTGTTCTAATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCA
TGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGA
ATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGT
CGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCA
AAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTC
ATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAG
ATTCACGTGATGGATTGCATCATTTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTKNHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLN
SSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSA
DGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTS
NRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTK
LSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESML
SEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVD
RAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQME
VYCSNFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQS
RHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQ
IHVMDCII*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTKNHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLN
SSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSA
DGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTS
NRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTK
LSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESML
SEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVD
RAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQME
VYCSNFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQS
RHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQ
IHVMDCII*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression with N term GST.
100042 R 1000 1001 1047 19007
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