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Plasmid
pEBG6P-1 ITCH R446A C830A
Parent Plasmid
pEBG6P
Expressed
GST-ITCH R446A C830A
DU Number
DU31307
Genbank
NM_031483
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA
GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG
ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC
AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC
CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG
AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA
TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG
ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA
AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC
TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC
AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA
GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA
ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT
ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA
AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT
TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA
CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA
CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT
TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGGCATTCACAGTGGAT
GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG
TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA
CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT
TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA
GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT
GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT
GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT
GGCAGATTCATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG
TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA
AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT
AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA
CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG
GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT
TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG
CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT
GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA
AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA
TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA
TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCGCTTTTAATCGCC
TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG
TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSDSGSQLGSMGS
LTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSP
KWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKL
EEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQN
DDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPP
RPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSG
LIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTW
DRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQR
SQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRV
YFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMAFTVDGIPYFVDHNRRTT
TYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTR
KTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEV
LNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKF
IDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMY
FSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQT
QAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYAR
TSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKF
CIEKVGKENWLPRSHTAFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE*

Vector Type
Mammalian
Antibiotic
Amp
Comments
Nucleotide sequence difference compared with NM_031483 CDS:
t1809c (silent in F603)
Mammalian expression plasmid to express Itch with N-terminal GST (contains preScission protease site)

Estimated MW of expressed protein : 126091 Daltons
100042 R 1000 1001 1047 19007
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