USP22 | Ubiquitylation Resource

Plasmid

pFB.HTb USP22

Parent Plasmid

pFastBac HTb

Expressed

6His USP22

DU Number

DU36274

Genbank

NM_015276.1 GI:150010638

Tag

6His

Species

Human

Sequence of Insert

View Sequence

ggatccatggtgtcccggccagagcccgagggcgaggccatggacgccga
gctggcggtagcgccgccgggctgctcgcacctgggcagcttcaaggtgg
acaactggaagcagaacctgcgggccatctaccagtgcttcgtgtggagc
ggcacggctgaggcccgcaagcgcaaggccaagtcctgtatctgccatgt
ctgtggcgtccacctcaacaggctgcattcctgcctctactgtgtcttct
tcggctgtttcacaaagaagcatattcacgagcatgcgaaggcgaagcgg
cacaacctggccattgatctgatgtacggaggcatctactgttttctgtg
ccaggactacatctatgacaaagacatggaaataatcgccaaggaggagc
agcgaaaagcttggaaaatgcaaggcgttggagagaagttttcaacttgg
gaaccaaccaaacgggagcttgaactgctgaagcacaacccgaaaaggag
aaagatcacctcgaactgcaccataggtctgcgtgggctgatcaaccttg
ggaacacatgcttcatgaactgcatcgtgcaggccctgacccacacgcca
cttctgcgggacttcttcctgtctgacaggcaccgctgtgagatgcagag
ccccagctcctgtctggtctgtgagatgtcctcactgtttcaggagtttt
actctggacaccggtcccctcacatcccgtataagttgctgcacctggtg
tggacccacgcgaggcacctagcaggctacgagcagcaggacgcccacga
gttcctcatcgcggccctggacgtgctccaccgacactgcaaaggtgatg
acaatgggaagaaggccaacaaccccaaccactgcaactgcatcatagac
cagatcttcacaggcgggttgcagtcagacgtcacctgccaagtctgcca
tggagtctccaccaccatcgaccccttctgggacatcagcttggatctcc
ccggctcttccaccccattctggcccctgagcccagggagcgagggcaac
gtggtaaacggggaaagccacgtgtcgggaaccaccacgctcacggactg
cctgcgacgattcaccagaccagagcacttgggcagcagcgccaagatca
agtgcagcggttgccatagctaccaggagtccacaaagcagctcactatg
aagaaactgcccatcgtagcctgttttcatctcaaacgatttgaacactc
agccaagctgcggcggaagatcaccacgtatgtgtccttccccctggagc
tggacatgacccctttcatggcctccagcaaagagagcaggatgaatgga
cagtaccagcagcccacggacagtctcaacaatgacaacaagtattccct
gtttgctgttgttaaccatcaagggaccttggagagtggccactacacca
gctttatccggcagcacaaagaccagtggttcaagtgtgacgatgccatc
atcaccaaggccagcatcaaggacgtcctggacagcgaagggtacttgct
gttctatcacaaacagttcctggaatacgagtaggtcgactcgagcggcc
gc

Amino Acid Sequence

View Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMVSRPEPEGEAMDAELAVAPPG
CSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVHLNR
LHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDK
DMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCT
IGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSCLVC
EMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIAALD
VLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTID
PFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRP
EHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKI
TTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQ
GTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFL
EYE*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

Baculovirus expression plasmid with N terminal 6His tag

Estimated MW of expressed protein : 63330 Daltons

100042 R 1000 1001 1047 19007