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Plasmid
pETDuet-1 6-His Precission SAE2
Parent Plasmid
pETDuet1
Expressed
6His Precission SAE2
DU Number
DU20274
Tag
6His
Species
Human
Sequence of Insert
View Sequence
CCATGGCACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCAAT
GGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGGGG
GCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCAAG
AATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGATAC
TATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACATG
TTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTTAC
CCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGACTA
TAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGATA
ACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATGTT
CCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACTAT
CAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCAGA
GAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATACAT
TGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAAGA
TGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTGGG
AACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTGAC
ATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGATCC
AGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTTGA
CAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACTGG
GCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAGAA
TGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAGCT
ATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAGCA
GAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCTGC
AATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAGTA
TGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTATT
CCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTGGA
AGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTTTT
TGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCACTG
GATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTGAC
TGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAAGA
TAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAGAT
GGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCTAA
TAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCTTC
AAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTCAT
AGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCCCC
GGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAACCA
ATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGCAA
GATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAATGC
CGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAAAG
AGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTGAT
GATGTCATAGCATTAGATTGA
GGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGGGG
GCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCAAG
AATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGATAC
TATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACATG
TTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTTAC
CCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGACTA
TAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGATA
ACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATGTT
CCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACTAT
CAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCAGA
GAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATACAT
TGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAAGA
TGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTGGG
AACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTGAC
ATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGATCC
AGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTTGA
CAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACTGG
GCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAGAA
TGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAGCT
ATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAGCA
GAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCTGC
AATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAGTA
TGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTATT
CCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTGGA
AGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTTTT
TGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCACTG
GATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTGAC
TGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAAGA
TAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAGAT
GGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCTAA
TAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCTTC
AAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTCAT
AGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCCCC
GGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAACCA
ATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGCAA
GATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAATGC
CGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAAAG
AGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTGAT
GATGTCATAGCATTAGATTGA
Amino Acid Sequence
View Sequence
PWHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD
Vector Type
Bacterial
Antibiotic
Amp
Comments
Estimated MW of expressed protein : 73198 Daltons
100042 R 1000 1001 1047 19007
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