NEMO | Ubiquitylation Resource

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Plasmid

pEBG6P-1 NEMO R319Q

Parent Plasmid

pEBG6P

Expressed

GST-NEMO R319Q

DU Number

DU4832

Genbank

AAH50612

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTG
CAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTC
TCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTC
CTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCC
ATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCA
TTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCC
AGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTG
AAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATG
CCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGT
CCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACT
AAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGC
GCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGC
AGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGC
ATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCA
GGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCA
GCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAA
CAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGAT
CGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCG
TTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCT
GAGAGGCAGGCCCAGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGA
GCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTC
AGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCC
CAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCT
GCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTC
CCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTC
ATGGAGTGCATTGAGTAGGCGGCCGCG

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSNRHLWKSQLCEMV
QPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDA
IRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDL
KRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAAT
KECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALR
MERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLK
QQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQA
ERQAQEKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVS
QAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHV
MECIE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Mammalian expression with N term GST.

100042 R 1000 1001 1047 19007

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