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Plasmid
pCMV5-FLAG-1 Cullin2 K689R
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG Cul2 K689R
DU Number
DU20288
Genbank
NM_003591
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAA
CAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAA
GAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCC
TATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTT
GGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAAC
AAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCA
GACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAA
GAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGG
AAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCG
AGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCC
ATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTC
CCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAAC
AGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACT
GCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATT
CGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGA
ATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAAT
GTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTAC
GTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCT
GCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTC
AGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGT
AAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTAT
GAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTA
AGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTA
CTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCT
CACGAGCTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTC
AAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCC
ATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTG
TGGTTATGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTG
TCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGAC
ACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGG
TGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGG
AATTAGAAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTC
AGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAA
AATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAA
TGGCAGTTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAG
CTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAA
ATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATA
TTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGA
ACAAAATTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAAT
GGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAG
CTGCTATAGTTCGTATCATGAGAGCACGAAAAGTGCTTCGGCACAATGCC
CTTATTCAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTAT
CAGCATGATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAG
AACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCC
GC
CAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAA
GAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCC
TATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTT
GGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAAC
AAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCA
GACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAA
GAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGG
AAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCG
AGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCC
ATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTC
CCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAAC
AGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACT
GCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATT
CGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGA
ATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAAT
GTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTAC
GTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCT
GCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTC
AGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGT
AAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTAT
GAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTA
AGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTA
CTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCT
CACGAGCTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTC
AAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCC
ATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTG
TGGTTATGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTG
TCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGAC
ACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGG
TGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGG
AATTAGAAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTC
AGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAA
AATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAA
TGGCAGTTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAG
CTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAA
ATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATA
TTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGA
ACAAAATTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAAT
GGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAG
CTGCTATAGTTCGTATCATGAGAGCACGAAAAGTGCTTCGGCACAATGCC
CTTATTCAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTAT
CAGCATGATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAG
AACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCC
GC
Amino Acid Sequence
View Sequence
DYKDDDDKGSMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMRARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA
Vector Type
Mammalian
Antibiotic
Amp
Comments
Estimated MW of expressed protein : 88151.2
100042 R 1000 1001 1047 19007
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