MEKK1 | Ubiquitylation Resource

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Synonyms

MAP3K1

Plasmid

pGEX4T-1-MEKK1 delta 1-662 6His

Parent Plasmid

pGEX4T1

Expressed

GST-MEKK1 delta 1-662 6His

DU Number

DU5076

Genbank

XM_042066

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ggatccATGGTTACTACAGTACCCCATGTGTTTTCAAAACTGTTAGAAAT
GCTGAGTGTTTCCAGTTCCACTCACTTCACCAGGATGCGTCGCCGTTTGA
TGGCTATTGCAGATGAGGTGGAAATTGCCGAAGCCATCCAGTTGGGCGTA
GAAGACACTTTGGATGGTCAACAGGACAGCTTCTTGCAGGCATCTGTTCC
CAACAACTATCTGGAAACCACAGAGAACAGTTCCCCTGAGTGCACAATCC
ATTTAGAGAAAACTGGAAAAGGATTATGTGCTACAAAATTGAGTGCCAGT
TCAGAGGACATTTCTGAGAGACTGGCCAGCATTTCAGTAGGACCTTCTAG
TTCAACAACAACAACAACAACAACAGAGCAACCAAAGCCAATGGTTCAAA
CAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTATCTCAT
CATTCCCAATTAATGTTTCCAGCCTTGTCAACCCCTTCTTCTTCTACCCC
ATCTGTACCAGCTGGCACTGCAACAGATGTCTCTAAGCATAGACTTCAGG
GATTCATTCCCTGCAGAATACCTTCTGCATCTCCTCAAACACAGCGCAAG
TTTTCTCTACAATTCCACAGAAACTGTCCTGAAAACAAAGACTCAGATAA
ACTTTCCCCAGTCTTTACTCAGTCAAGACCCTTGCCCTCCAGTAACATAC
ACAGGCCAAAGCCATCTCGACCTACCCCAGGTAATACAAGTAAACAGGGA
GATCCCTCAAAAAATAGCATGACACTTGATCTGAACAGTAGTTCCAAATG
TGATGACAGCTTTGGCTGTAGCAGCAATAGTAGTAATGCTGTTATACCCA
GTGACGAGACAGTGTTCACCCCAGTAGAGGAGAAATGCAGATTAGATGTC
AATACAGAGCTCAACTCCAGTATTGAGGACCTTCTTGAAGCATCTATGCC
TTCAAGTGATACAACAGTAACTTTTAAGTCAGAAGTTGCTGTCCTGTCTC
CTGAAAAGGCTGAAAATGATGATACCTACAAAGATGATGTGAATCATAAT
CAAAAGTGCAAAGAGAAGATGGAAGCTGAAGAAGAAGAAGCTTTAGCAAT
TGCCATGGCAATGTCAGCGTCTCAGGATGCCCTCCCCATAGTTCCTCAGC
TGCAGGTTGAAAATGGAGAAGATATCATCATTATTCAACAGGATACACCA
GAGACTCTACCAGGACATACCAAAGCAAAACAACCGTATAGAGAAGACAC
TGAATGGCTGAAAGGTCAACAGATAGGCCTTGGAGCATTTTCTTCTTGTT
ATCAGGCTCAAGATGTGGGAACTGGAACTTTAATGGCTGTTAAACAGGTG
ACTTATGTCAGAAACACATCTTCTGAGCAAGAAGAAGTAGTAGAAGCACT
AAGAGAAGAGATAAGAATGATGAGCCATCTGAATCATCCAAACATCATTA
GGATGTTGGGAGCCACGTGTGAGAAGAGCAATTACAATCTCTTCATTGAA
TGGATGGCAGGGGGATCGGTGGCTCATTTGCTGAGTAAATATGGAGCCTT
CAAAGAATCAGTAGTTATTAACTACACTGAACAGTTACTCCGTGGCCTTT
CGTATCTCCATGAAAACCAAATCATTCACAGAGATGTCAAAGGTGCCAAT
TTGCTAATTGACAGCACTGGTCAGAGACTAAGAATTGCAGATTTTGGAGC
TGCAGCCAGGTTGGCATCAAAAGGAACTGGTGCAGGAGAGTTTCAGGGAC
AATTACTGGGGACAATTGCATTTATGGCACCTGAGGTACTAAGAGGTCAA
CAGTATGGAAGGAGCTGTGATGTATGGAGTGTTGGCTGTGCTATTATAGA
AATGGCTTGTGCAAAACCACCATGGAATGCAGAAAAACACTCCAATCATC
TTGCTTTGATATTTAAGATTGCTAGTGCAACTACTGCTCCATCGATCCCT
TCACATTTGTCTCCTGGTTTACGAGATGTGGCTCTTCGTTGTTTAGAACT
TCAACCTCAGGACAGACCTCCATCAAGAGAGCTACTGAAGCATCCAGTCT
TTCGTACTACATGGCATCATCACCATCACCATTGAgaattc

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVTTVPHVFSKLLEMLSVSSSTHF
TRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTEN
SSPECTIHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTE
QPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATD
VSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSR
PLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSN
SSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFK
SEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQD
ALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIG
LGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSH
LNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYT
EQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGT
GAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWN
AEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSR
ELLKHPVFRTTWHHHHHH*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial construct to express MEKK1 delta 1-662 with N terminal GST and C terminal 6His.
Estimated MW of expressed protein : 102160 Daltons

100042 R 1000 1001 1047 19007

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