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Plasmid
pcDNA5-FRT/TO-HA- DCUN1D1 D211A A235R D241A
Parent Plasmid
pcDNA5 FRT/TO HA1
Expressed
HA-DCUN1D1 D211A A235R D241A
DU Number
DU23570
Tag
HA
Species
Human
Sequence of Insert
View Sequence
ggatccATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGcCACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAagATGGCCTGTTCTTATTGcTGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGcCACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAagATGGCCTGTTCTTATTGcTGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLD
VATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQ
FCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIEKLK
AQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNG
RFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGRWPV
LIADFVEFARPQIAGTKSTTV*
VATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQ
FCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIEKLK
AQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNG
RFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGRWPV
LIADFVEFARPQIAGTKSTTV*
Vector Type
Mammalian
Antibiotic
Amp
Comments
a632c (D211A), g703a/c704g (A235R) and a722c (D241A). clone also harbours silent substitution g318a (Ala106) compared to NCBI ref
100042 R 1000 1001 1047 19007
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