TRAF3 | Ubiquitylation Resource

Plasmid

pCMV-FLAG TRAF3 (TNF receptor-associated factor 3 )

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG-TRAF3

DU Number

DU13782

Genbank

AAH75086.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGCGCTGCAGAC
TAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTT
TTGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAG
GACAAGTACAAGTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCA
GACCGAGTGTGGGCACCGCTTCTGCGAGAGCTGCATGGCGGCCCTGCTGA
GCTCTTCAAGTCCAAAATGTACAGCGTGTCAAGAGAGCATCGTTAAAGAT
AAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGAT
CTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGAC
ATCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGT
GTGCGTCCTGACTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCA
CGTGGAGAAGGCGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGA
GTCAGGTTCCGATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCC
TGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAG
GAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCT
GTAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATC
AAGGCCCACGAGGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGA
GTGGAGCAACTCGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTG
TAGAAAAAAACAAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTT
GAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAA
AATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGG
AGCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGAC
AGCATGAAGAGCAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGA
GAGCGTGGACAAGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGG
AGTCCCAGCTGAGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGC
CTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAA
TGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGG
CCGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGT
TACTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGAT
GGGGAAGGGGACGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAAT
ATGATGCCCTGCTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTG
ATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGA
CCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCT
CTGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATAT
ATTAAAGATGATACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCT
GCCCGATCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGG
YKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKC
TACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLK
NDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIA
LQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYG
CVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSI
QSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIR
PFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRH
DQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTL
SLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPW
PFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFV
AQTVLENGTYIKDDTIFIKVIVDTSDLPDP

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter HA for mammalian expression.

Estimated MW of expressed protein : 65848 Daltons

100042 R 1000 1001 1047 19007