NEDD4

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Plasmid
pCMV-HA-1 NEDD4
Parent Plasmid
pCMV5 HA1
Expressed
HA NEDD4
DU Number
DU32251
Genbank
NM_006154.2
Tag
HA
Species
Human
Sequence of Insert
GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC
Amino Acid Sequence
MYPYDVPDYAGSMATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILG
ASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLL
FEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKS
RVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQ
EPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQL
QAQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAFPSPPPS
SNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQP
TLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETS
QLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPF
FIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGR
IFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQND
IPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGV
AREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFI
GRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWI
LENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQ
WRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDW
REHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGF
AELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQM
AIENTQGFDGVD
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter HA for mammalian expression

Estimated MW of expressed protein : 105519 Daltons
100042 R 1000 1001 1047 19007
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