Nemo | Ubiquitylation Resource

Plasmid

pCMV-HA Nemo

Parent Plasmid

pCMV5 HA1

Expressed

HA-Nemo

DU Number

DU12632

Genbank

AAH50612

Tag

HA

Species

Human

Sequence of Insert

View Sequence

GGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCC
CAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTC
TGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAG
ACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCG
GCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCC
AAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAG
GCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAG
GCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGC
AGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTG
CTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGA
ATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGC
AGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTG
GACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGA
GCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGG
CCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTG
GTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCA
GCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATA
AGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCG
GTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAG
GCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGC
TGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAG
TCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGC
CCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCA
GCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAG
TGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGA
GTGCATTGAGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MYPYDVPDYAGSNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAML
HLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREE
KEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDK
ASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESERE
ALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQ
EYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAE
QHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQRE
YSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPP
EEPPDFCCPKCQYQAPDMDTLQIHVMECIE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter HA for mammalian expression.

Estimated MW of expressed protein : 49423 Daltons

100042 R 1000 1001 1047 19007