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NEMO

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Plasmid
pCMV-TAG3b NEMO L327P
Parent Plasmid
pCMV tag3b
Expressed
Myc-NEMO L327P
DU Number
DU8686
Genbank
AAH50612
Tag
Myc
Species
Human
Sequence of Insert
GGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCC
CAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTC
TGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAG
ACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCG
GCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCC
AAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAG
GCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAG
GCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGC
AGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTG
CTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGA
ATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGC
AGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTG
GACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGA
GCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGG
CCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTG
GTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCA
GCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATA
AGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCG
GTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAG
GCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCCGCAGGAGCAGC
TGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAG
TCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGC
CCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCA
GCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAG
TGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGA
GTGCATTGAGTAGGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTCGAG
Amino Acid Sequence
MEQKLISEEDLSPGGSNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGK
PAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQAS
QREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQM
AEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLE
SEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYH
QLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLK
EEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELPQEQLEQ
LQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQR
RSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE*
Vector Type
Mammalian
Antibiotic
Kan
Comments
N terminal Myc tag for mammalian expression.

Estimated MW of expressed protein : 49750 Daltons
100042 R 1000 1001 1047 19007
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