BIRC3 | Ubiquitylation Resource

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Plasmid

pEBG6P-BIRC3

Parent Plasmid

pEBG6P

Expressed

GST BIRC3

DU Number

DU32415

Genbank

NP_001156.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GATCCATGAACATAGTAGAAAACAGCATATTCTTATCAAATTTGATGAAA
AGCGCCAACACGTTTGAACTGAAATACGACTTGTCATGTGAACTGTACCG
AATGTCTACGTATTCCACTTTTCCTGCTGGGGTTCCTGTCTCAGAAAGGA
GTCTTGCTCGTGCTGGTTTCTATTACACTGGTGTGAATGACAAGGTCAAA
TGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAAAGAGGAGACAGTCC
TACTGAAAAGCATAAAAAGTTGTATCCTAGCTGCAGATTCGTTCAGAGTC
TAAATTCCGTTAACAACTTGGAAGCTACCTCTCAGCCTACTTTTCCTTCT
TCAGTAACAAATTCCACACACTCATTACTTCCGGGTACAGAAAACAGTGG
ATATTTCCGTGGCTCTTATTCAAACTCTCCATCAAATCCTGTAAACTCCA
GAGCAAATCAAGATTTTTCTGCCTTGATGAGAAGTTCCTACCACTGTGCA
ATGAATAACGAAAATGCCAGATTACTTACTTTTCAGACATGGCCATTGAC
TTTTCTGTCGCCAACAGATCTGGCAAAAGCAGGCTTTTACTACATAGGAC
CTGGAGACAGAGTGGCTTGCTTTGCCTGTGGTGGAAAATTGAGCAATTGG
GAACCGAAGGATAATGCTATGTCAGAACACCTGAGACATTTTCCCAAATG
CCCATTTATAGAAAATCAGCTTCAAGACACTTCAAGATACACAGTTTCTA
ATCTGAGCATGCAGACACATGCAGCCCGCTTTAAAACATTCTTTAACTGG
CCCTCTAGTGTTCTAGTTAATCCTGAGCAGCTTGCAAGTGCGGGTTTTTA
TTATGTGGGTAACAGTGATGATGTCAAATGCTTTTGCTGTGATGGTGGAC
TCAGGTGTTGGGAATCTGGAGATGATCCATGGGTTCAACATGCCAAGTGG
TTTCCAAGGTGTGAGTACTTGATAAGAATTAAAGGACAGGAGTTCATCCG
TCAAGTTCAAGCCAGTTACCCTCATCTACTTGAACAGCTGCTATCCACAT
CAGACAGCCCAGGAGATGAAAATGCAGAGTCATCAATTATCCATTTTGAA
CCTGGAGAAGACCATTCAGAAGATGCAATCATGATGAATACTCCTGTGAT
TAATGCTGCCGTGGAAATGGGCTTTAGTAGAAGCCTGGTAAAACAGACAG
TTCAGAGAAAAATCCTAGCAACTGGAGAGAATTATAGACTAGTCAATGAT
CTTGTGTTAGACTTACTCAATGCAGAAGATGAAATAAGGGAAGAGGAGAG
AGAAAGAGCAACTGAGGAAAAAGAATCAAATGATTTATTATTAATCCGGA
AGAATAGAATGGCACTTTTTCAACATTTGACTTGTGTAATTCCAATCCTG
GATAGTCTACTAACTGCCGGAATTATTAATGAACAAGAACATGATGTTAT
TAAACAGAAGACACAGACGTCTTTACAAGCAAGAGAACTGATTGATACGA
TTTTAGTAAAAGGAAATATTGCAGCCACTGTATTCAGAAACTCTCTGCAA
GAAGCTGAAGCTGTGTTATATGAGCATTTATTTGTGCAACAGGACATAAA
ATATATTCCCACAGAAGATGTTTCAGATCTACCAGTGGAAGAACAATTGC
GGAGACTACAAGAAGAAAGAACATGTAAAGTGTGTATGGACAAAGAAGTG
TCCATAGTGTTTATTCCTTGTGGTCATCTAGTAGTATGCAAAGATTGTGC
TCCTTCTTTAAGAAAGTGTCCTATTTGTAGGAGTACAATCAAGGGTACAG
TTCGTACATTTCTTTCATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNIVENSIFLSNL
MKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDK
VKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTF
PSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYH
CAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLS
NWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFF
NWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHA
KWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIH
FEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLV
NDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIP
ILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNS
LQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDK
EVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression

Estimated MW of expressed protein : 95727 Daltons

100042 R 1000 1001 1047 19007

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