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Nemo

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Plasmid
pcDNA5-FRT/TO Nemo FLAG
Parent Plasmid
pcDNA5 FRT/TO
Expressed
Nemo FLAG
DU Number
DU12840
Genbank
AAH50612
Tag
FLAG
Species
Human
Sequence of Insert
GGATCCGCCACCATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGAT
GGTGCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAG
AGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGC
GCTCCTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGA
TGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTC
TGCATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAG
TTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGA
TCTGAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGA
GATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTG
ACGTCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGC
CACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGC
AGGCGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGC
GTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCT
CCGCATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGT
TGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAG
AGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCT
CAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGG
TGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAG
ACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCA
GGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGC
AGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGC
TGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGT
CTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGG
CCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGC
TGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACA
TGTCATGGAGTGCATTGAGGACTACAAGGACGATGACGATAAGTAAGCGG
CCGC
Amino Acid Sequence
MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPET
LQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEA
RKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLL
GELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVD
QLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVV
GSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPV
LKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQES
ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKC
QYQAPDMDTLQIHVMECIEDYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
C ter FLAG for mammalian expression.

Estimated MW of expressed protein : 49190 Daltons
100042 R 1000 1001 1047 19007
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