CBL | Ubiquitylation Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pGEX6P-1-CBL

Parent Plasmid

pGEX6P1

Expressed

GST-CBL

DU Number

DU12029

Genbank

P22681

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCCGGCAACGTGAAGAAGAGCTCTGGGGCCGGGGGCGGCAG
CGGCTCCGGGGGCTCGGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACG
CCTTCCAGCCGCACCACCACCACCACCACCACCTCAGCCCCCACCCGCCG
GGGACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAA
GGTGGTGCGGTTGTGTCAGAACCCAAAGCTGGCGCTAAAGAATAGCCCAC
CTTATATCTTAGACCTGCTACCAGATACCTACCAGCATCTCCGTACTATC
TTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATTT
TAGGGTGTTTATGGAGAATTTGATGAAGAAAACTAAGCAAACCATAAGCC
TCTTCAAGGAGGGAAAAGAAAGAATGTATGAGGAGAATTCTCAGCCTAGG
CGAAACCTAACCAAACTGTCCCTCATCTTCAGCCACATGCTGGCAGAACT
AAAAGGAATCTTTCCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTA
CTAAAGCAGATGCTGCGGAATTTTGGAGAAAAGCTTTTGGGGAAAAGACA
ATAGTCCCTTGGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCAT
CAGTTCTGGGCTGGAGGCCATGGCTCTGAAATCCACTATTGATCTGACCT
GCAATGATTATATTTCGGTTTTTGAATTTGACATCTTTACCCGACTCTTT
CAGCCCTGGTCCTCTTTGCTCAGGAATTGGAACAGCCTTGCTGTAACTCA
TCCTGGCTACATGGCTTTTTTGACGTATGACGAAGTGAAAGCTCGGCTCC
AGAAATTCATTCACAAACCTGGCAGTTATATCTTCCGGCTGAGCTGTACT
CGTCTGGGTCAGTGGGCTATTGGGTATGTTACTGCTGATGGGAACATTCT
CCAGACAATCCCTCACAATAAACCTCTCTTCCAAGCACTGATTGATGGCT
TCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAGAATCCTGAT
CTGACTGGCTTATGTGAACCAACTCCCCAAGACCATATCAAAGTGACCCA
GGAACAATATGAATTATACTGTGAGATGGGCTCCACATTCCAACTATGTA
AAATATGTGCTGAAAATGATAAGGATGTAAAGATTGAGCCCTGTGGACAC
CTCATGTGCACATCCTGTCTTACATCCTGGCAGGAATCAGAAGGTCAGGG
CTGTCCTTTCTGCCGATGTGAAATTAAAGGTACTGAACCCATCGTGGTAG
ATCCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAG
GGAGCTCCCTCCCCAAATTATGATGATGATGATGATGAACGAGCTGATGA
TACTCTCTTCATGATGAAGGAATTGGCTGGTGCCAAGGTGGAACGGCCGC
CTTCTCCATTCTCCATGGCCCCACAAGCTTCCCTTCCCCCGGTGCCACCA
CGACTTGACCTTCTGCCGCAGCGAGTATGTGTTCCCTCAAGTGCTTCTGC
TCTTGGAACTGCTTCTAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAAC
CATTGCCAGTACCTCCCACACTTCGAGATCTTCCACCACCACCGCCTCCA
GACCGGCCATATTCTGTTGGAGCAGAATCCCGACCTCAAAGACGCCCCTT
GCCTTGTACACCAGGCGACTGTCCCTCCAGAGACAAACTGCCCCCTGTCC
CCTCTAGCCGCCTTGGAGACTCATGGCTGCCCCGGCCAATCCCCAAAGTA
CCAGTATCTGCCCCAAGTTCCAGTGATCCCTGGACAGGAAGAGAATTAAC
CAACCGGCACTCACTTCCATTTTCATTGCCCTCACAAATGGAGCCCAGAC
CAGATGTGCCTAGGCTCGGAAGCACGTTCAGTCTGGATACCTCCATGAGT
ATGAATAGCAGCCCATTAGTAGGTCCAGAGTGTGACCACCCCAAAATCAA
ACCTTCCTCATCTGCCAATGCCATTTATTCTCTGGCTGCCAGACCTCTTC
CTGTGCCAAAACTGCCACCTGGGGAGCAATGTGAGGGTGAAGAGGACACA
GAGTACATGACTCCCTCTTCCAGGCCTCTACGGCCTTTGGATACATCCCA
GAGTTCACGAGCATGTGATTGCGACCAGCAGATTGATAGCTGTACGTATG
AAGCAATGTATAATATTCAGTCCCAGGCGCCATCTATCACCGAGAGCAGC
ACCTTTGGTGAAGGGAATTTGGCCGCAGCCCATGCCAACACTGGTCCCGA
GGAGTCAGAAAATGAGGATGATGGGTATGATGTCCCAAAGCCACCTGTGC
CGGCCGTGCTGGCCCGCCGAACTCTCTCAGATATCTCTAATGCCAGCTCC
TCCTTTGGCTGGTTGTCTCTGGATGGTGATCCTACAACAAATGTCACTGA
AGGTTCCCAAGTTCCCGAGAGGCCTCCAAAACCATTCCCGCGGAGAATCA
ACTCTGAACGGAAAGCTGGCAGCTGTCAGCAAGGTAGTGGTCCTGCCGCC
TCTGCTGCCACCGCCTCACCTCAGCTCTCCAGTGAGATCGAGAACCTCAT
GAGTCAGGGGTACTCCTACCAGGACATCCAGAAAGCTTTGGTCATTGCCC
AGAACAACATCGAGATGGCCAAAAACATCCTCCGGGAATTTGTTTCCATT
TCTTCTCCTGCCCATGTAGCTACCTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGNVKKSSGAGGGSGSGG
SGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRL
CQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFM
ENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIF
PSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGL
EAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYM
AFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIP
HNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYE
LYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFC
RCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFM
MKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTA
SKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTP
GDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHS
LPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSS
ANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRA
CDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESEN
EDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQV
PERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGY
SYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 126453 Daltons

100042 R 1000 1001 1047 19007

Download datasheet