IRAK2 | Ubiquitylation Resource

Plasmid

pFBHTb IRAK2

Parent Plasmid

pFastBac HTb

Expressed

6His IRAK2

DU Number

DU15203

Genbank

NM_001570.3

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGA
CCTGTGCCGCAACATGGACGCGCTCAGCGAGTGGGACTGGATGGAGTTCG
CCTCCTACGTGATCACAGACCTGACCCAGCTGCGGAAGATCAAGTCCATG
GAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTGCTGTGGTGGTGGGG
CATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGCCTGG
AGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAA
ATCAGGTGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCC
TTTGGCAGCTTCTGTAAGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGC
CTGTGAGGATGGCCACCTTTCCAGGCCCAGGGTCCTCTCCAGCCAGAGCC
CACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGATGCCCCTCATTCCTT
GAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACCTCCA
TTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGG
AGTGAGGCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAA
AATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGA
AGCCATTCGTCTTCAAGAAGCTCAGAGAGACAGCCTGTTCAAGTCCAGGA
TCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTG
CCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAGTTTC
ACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTG
CAGGGTCAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCAT
CTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCA
TCCACAGCAACGTCAAGAGCTCTAATGTCTTGCTGGACCAAAATCTCACC
CCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTC
AAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCGTATC
TGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATC
TTCAGCTGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAAT
GGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATA
TTCCAAGCAGCACCGCCTCGCTCTGCTCCAGGAAGACGGGCGTGGAGAAC
GTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAG
GCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTGTGCC
TGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCT
GTGGAAGAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCT
TTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACG
TTGACAATTCCAGCCTTGATGCCTCCTCCTCCATGAGTGTGGCACCCTGG
GCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCT
GCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGTGTTG
GCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATC
AATGAGGCCAAAAGGAAACTGATGGAGAATATTCTGCTCTACAAAGAGGA
AAAAGTGGACAGCATTGAGCTCTTTGGCCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMACYIYQLPSWVLDDLCRNMDA
LSEWDWMEFASYVITDLTQLRKIKSMERVQGVSITRELLWWWGMRQATVQ
QLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRK
AEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTS
SDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFA
DVYRGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPV
LGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCA
VEYLHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKT
HLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPV
YLKDLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAE
ALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSS
SNTPEETDDVDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGRE
ADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIEL
FGP*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

N ter 6HIS for baculovirus expression.

Estimated MW of expressed protein : 72802 Daltons

100042 R 1000 1001 1047 19007