CBL | Ubiquitylation Resource

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Plasmid

pEBG6P-CBL

Parent Plasmid

pEBG6P

Expressed

GST-CBL

DU Number

DU8350

Genbank

P22681

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCCGGCAACGTGAAGAAGAGCTCTGGGGCCGGGGGCGGCAG
CGGCTCCGGGGGCTCGGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACG
CCTTCCAGCCGCACCACCACCACCACCACCACCTCAGCCCCCACCCGCCG
GGGACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAA
GGTGGTGCGGTTGTGTCAGAACCCAAAGCTGGCGCTAAAGAATAGCCCAC
CTTATATCTTAGACCTGCTACCAGATACCTACCAGCATCTCCGTACTATC
TTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATTT
TAGGGTGTTTATGGAGAATTTGATGAAGAAAACTAAGCAAACCATAAGCC
TCTTCAAGGAGGGAAAAGAAAGAATGTATGAGGAGAATTCTCAGCCTAGG
CGAAACCTAACCAAACTGTCCCTCATCTTCAGCCACATGCTGGCAGAACT
AAAAGGAATCTTTCCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTA
CTAAAGCAGATGCTGCGGAATTTTGGAGAAAAGCTTTTGGGGAAAAGACA
ATAGTCCCTTGGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCAT
CAGTTCTGGGCTGGAGGCCATGGCTCTGAAATCCACTATTGATCTGACCT
GCAATGATTATATTTCGGTTTTTGAATTTGACATCTTTACCCGACTCTTT
CAGCCCTGGTCCTCTTTGCTCAGGAATTGGAACAGCCTTGCTGTAACTCA
TCCTGGCTACATGGCTTTTTTGACGTATGACGAAGTGAAAGCTCGGCTCC
AGAAATTCATTCACAAACCTGGCAGTTATATCTTCCGGCTGAGCTGTACT
CGTCTGGGTCAGTGGGCTATTGGGTATGTTACTGCTGATGGGAACATTCT
CCAGACAATCCCTCACAATAAACCTCTCTTCCAAGCACTGATTGATGGCT
TCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAGAATCCTGAT
CTGACTGGCTTATGTGAACCAACTCCCCAAGACCATATCAAAGTGACCCA
GGAACAATATGAATTATACTGTGAGATGGGCTCCACATTCCAACTATGTA
AAATATGTGCTGAAAATGATAAGGATGTAAAGATTGAGCCCTGTGGACAC
CTCATGTGCACATCCTGTCTTACATCCTGGCAGGAATCAGAAGGTCAGGG
CTGTCCTTTCTGCCGATGTGAAATTAAAGGTACTGAACCCATCGTGGTAG
ATCCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAG
GGAGCTCCCTCCCCAAATTATGATGATGATGATGATGAACGAGCTGATGA
TACTCTCTTCATGATGAAGGAATTGGCTGGTGCCAAGGTGGAACGGCCGC
CTTCTCCATTCTCCATGGCCCCACAAGCTTCCCTTCCCCCGGTGCCACCA
CGACTTGACCTTCTGCCGCAGCGAGTATGTGTTCCCTCAAGTGCTTCTGC
TCTTGGAACTGCTTCTAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAAC
CATTGCCAGTACCTCCCACACTTCGAGATCTTCCACCACCACCGCCTCCA
GACCGGCCATATTCTGTTGGAGCAGAATCCCGACCTCAAAGACGCCCCTT
GCCTTGTACACCAGGCGACTGTCCCTCCAGAGACAAACTGCCCCCTGTCC
CCTCTAGCCGCCTTGGAGACTCATGGCTGCCCCGGCCAATCCCCAAAGTA
CCAGTATCTGCCCCAAGTTCCAGTGATCCCTGGACAGGAAGAGAATTAAC
CAACCGGCACTCACTTCCATTTTCATTGCCCTCACAAATGGAGCCCAGAC
CAGATGTGCCTAGGCTCGGAAGCACGTTCAGTCTGGATACCTCCATGAGT
ATGAATAGCAGCCCATTAGTAGGTCCAGAGTGTGACCACCCCAAAATCAA
ACCTTCCTCATCTGCCAATGCCATTTATTCTCTGGCTGCCAGACCTCTTC
CTGTGCCAAAACTGCCACCTGGGGAGCAATGTGAGGGTGAAGAGGACACA
GAGTACATGACTCCCTCTTCCAGGCCTCTACGGCCTTTGGATACATCCCA
GAGTTCACGAGCATGTGATTGCGACCAGCAGATTGATAGCTGTACGTATG
AAGCAATGTATAATATTCAGTCCCAGGCGCCATCTATCACCGAGAGCAGC
ACCTTTGGTGAAGGGAATTTGGCCGCAGCCCATGCCAACACTGGTCCCGA
GGAGTCAGAAAATGAGGATGATGGGTATGATGTCCCAAAGCCACCTGTGC
CGGCCGTGCTGGCCCGCCGAACTCTCTCAGATATCTCTAATGCCAGCTCC
TCCTTTGGCTGGTTGTCTCTGGATGGTGATCCTACAACAAATGTCACTGA
AGGTTCCCAAGTTCCCGAGAGGCCTCCAAAACCATTCCCGCGGAGAATCA
ACTCTGAACGGAAAGCTGGCAGCTGTCAGCAAGGTAGTGGTCCTGCCGCC
TCTGCTGCCACCGCCTCACCTCAGCTCTCCAGTGAGATCGAGAACCTCAT
GAGTCAGGGGTACTCCTACCAGGACATCCAGAAAGCTTTGGTCATTGCCC
AGAACAACATCGAGATGGCCAAAAACATCCTCCGGGAATTTGTTTCCATT
TCTTCTCCTGCCCATGTAGCTACCTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAGNVKKSSGAGG
GSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLM
DKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENE
YFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLA
ELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVH
PISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAV
THPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGN
ILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKV
TQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEG
QGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERA
DDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSA
SALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRR
PLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRE
LTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPK
IKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDT
SQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTG
PEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNV
TEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIEN
LMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression.

Estimated MW of expressed protein : 127046 Daltons

100042 R 1000 1001 1047 19007

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