CBL

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Plasmid
pET156P CBL
Parent Plasmid
pET15b 6His 3C
Expressed
6His CBL
DU Number
DU12793
Genbank
P22681
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGCCGGCAACGTGAAGAAGAGCTCTGGGGCCGGGGGCGGCAG
CGGCTCCGGGGGCTCGGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACG
CCTTCCAGCCGCACCACCACCACCACCACCACCTCAGCCCCCACCCGCCG
GGGACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAA
GGTGGTGCGGTTGTGTCAGAACCCAAAGCTGGCGCTAAAGAATAGCCCAC
CTTATATCTTAGACCTGCTACCAGATACCTACCAGCATCTCCGTACTATC
TTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATTT
TAGGGTGTTTATGGAGAATTTGATGAAGAAAACTAAGCAAACCATAAGCC
TCTTCAAGGAGGGAAAAGAAAGAATGTATGAGGAGAATTCTCAGCCTAGG
CGAAACCTAACCAAACTGTCCCTCATCTTCAGCCACATGCTGGCAGAACT
AAAAGGAATCTTTCCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTA
CTAAAGCAGATGCTGCGGAATTTTGGAGAAAAGCTTTTGGGGAAAAGACA
ATAGTCCCTTGGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCAT
CAGTTCTGGGCTGGAGGCCATGGCTCTGAAATCCACTATTGATCTGACCT
GCAATGATTATATTTCGGTTTTTGAATTTGACATCTTTACCCGACTCTTT
CAGCCCTGGTCCTCTTTGCTCAGGAATTGGAACAGCCTTGCTGTAACTCA
TCCTGGCTACATGGCTTTTTTGACGTATGACGAAGTGAAAGCTCGGCTCC
AGAAATTCATTCACAAACCTGGCAGTTATATCTTCCGGCTGAGCTGTACT
CGTCTGGGTCAGTGGGCTATTGGGTATGTTACTGCTGATGGGAACATTCT
CCAGACAATCCCTCACAATAAACCTCTCTTCCAAGCACTGATTGATGGCT
TCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAGAATCCTGAT
CTGACTGGCTTATGTGAACCAACTCCCCAAGACCATATCAAAGTGACCCA
GGAACAATATGAATTATACTGTGAGATGGGCTCCACATTCCAACTATGTA
AAATATGTGCTGAAAATGATAAGGATGTAAAGATTGAGCCCTGTGGACAC
CTCATGTGCACATCCTGTCTTACATCCTGGCAGGAATCAGAAGGTCAGGG
CTGTCCTTTCTGCCGATGTGAAATTAAAGGTACTGAACCCATCGTGGTAG
ATCCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAG
GGAGCTCCCTCCCCAAATTATGATGATGATGATGATGAACGAGCTGATGA
TACTCTCTTCATGATGAAGGAATTGGCTGGTGCCAAGGTGGAACGGCCGC
CTTCTCCATTCTCCATGGCCCCACAAGCTTCCCTTCCCCCGGTGCCACCA
CGACTTGACCTTCTGCCGCAGCGAGTATGTGTTCCCTCAAGTGCTTCTGC
TCTTGGAACTGCTTCTAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAAC
CATTGCCAGTACCTCCCACACTTCGAGATCTTCCACCACCACCGCCTCCA
GACCGGCCATATTCTGTTGGAGCAGAATCCCGACCTCAAAGACGCCCCTT
GCCTTGTACACCAGGCGACTGTCCCTCCAGAGACAAACTGCCCCCTGTCC
CCTCTAGCCGCCTTGGAGACTCATGGCTGCCCCGGCCAATCCCCAAAGTA
CCAGTATCTGCCCCAAGTTCCAGTGATCCCTGGACAGGAAGAGAATTAAC
CAACCGGCACTCACTTCCATTTTCATTGCCCTCACAAATGGAGCCCAGAC
CAGATGTGCCTAGGCTCGGAAGCACGTTCAGTCTGGATACCTCCATGAGT
ATGAATAGCAGCCCATTAGTAGGTCCAGAGTGTGACCACCCCAAAATCAA
ACCTTCCTCATCTGCCAATGCCATTTATTCTCTGGCTGCCAGACCTCTTC
CTGTGCCAAAACTGCCACCTGGGGAGCAATGTGAGGGTGAAGAGGACACA
GAGTACATGACTCCCTCTTCCAGGCCTCTACGGCCTTTGGATACATCCCA
GAGTTCACGAGCATGTGATTGCGACCAGCAGATTGATAGCTGTACGTATG
AAGCAATGTATAATATTCAGTCCCAGGCGCCATCTATCACCGAGAGCAGC
ACCTTTGGTGAAGGGAATTTGGCCGCAGCCCATGCCAACACTGGTCCCGA
GGAGTCAGAAAATGAGGATGATGGGTATGATGTCCCAAAGCCACCTGTGC
CGGCCGTGCTGGCCCGCCGAACTCTCTCAGATATCTCTAATGCCAGCTCC
TCCTTTGGCTGGTTGTCTCTGGATGGTGATCCTACAACAAATGTCACTGA
AGGTTCCCAAGTTCCCGAGAGGCCTCCAAAACCATTCCCGCGGAGAATCA
ACTCTGAACGGAAAGCTGGCAGCTGTCAGCAAGGTAGTGGTCCTGCCGCC
TCTGCTGCCACCGCCTCACCTCAGCTCTCCAGTGAGATCGAGAACCTCAT
GAGTCAGGGGTACTCCTACCAGGACATCCAGAAAGCTTTGGTCATTGCCC
AGAACAACATCGAGATGGCCAAAAACATCCTCCGGGAATTTGTTTCCATT
TCTTCTCCTGCCCATGTAGCTACCTAGGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSMAGNVKKSSGAGGGSGSGGSGSGGLIG
LMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLAL
KNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTK
QTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGD
TFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKST
IDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEV
KARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQA
LIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGST
FQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTE
PIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAK
VERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSL
HKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDK
LPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQ
MEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLA
ARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQID
SCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVP
KPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPF
PRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKA
LVIAQNNIEMAKNILREFVSISSPAHVAT*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 102074 Daltons
100042 R 1000 1001 1047 19007
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